Quantitative Analysis of Free 15-F2t-Isoprostane from Plasma of Obstructive Sleep Apnea Patients Using Enzyme Linked Immunosorbent Assay Method with Immunoaffinity Extraction Technique
Journal Title: Indonesian Journal of Clinical Pharmacy - Year 2012, Vol 1, Issue 1
Abstract
15-F2t-isoprostane is a biomarker in assessment of oxidative stress status that due to its relatively low concentration in biological fluid and also has many isomers, the 15-F2t-isoprostane sample need to be extracted prior to the quantifying processes. Extraction techniques commonly used to extract 15-F2t-isoprostane are solid phase extraction (SPE) and immunoaffinity extraction. Improvements to the SPE and immunoaffinity extraction techniques had been conducted, and the recovery results was then compared. The quantification of 15-F2t-isoprostane then was conducted using Enzyme Linked Immunosorbent Assay (ELISA) method. Then followed by the examination of the plasma recovery results. Extraction technique which had the highest recovery then was used to quantify 15-F2t-isoprostane from plasma of Obstructive Sleep Apnea (OSA) patients. Immunoaffinity extraction technique has a good recovery result. OSA patients have the tendency to have high 15-F2t-isoprostane concentrations in the plasma, therefore have a potential risk to get diseases related to the biological activities of 15-F2t-isoprostane, such as arteriosclerosis.
Authors and Affiliations
Bertha Rusdi, Tutus Gusdinar, Miswar Fattah
Keywords
Related Articles
- EP ID EP333642
- DOI -
- Views 113
- Downloads 0
Lactobacillus plantarum as Biopreservative Agent in Paneer for Dietary Food of Diabetic and Coronary Heart Disease Patients
Paneer is a food product that is obtained by heating milk followed by acid coagulation. It is known as a diet food that is recommended for diabetic and coronary heart disease patients. A relatively shorter shelf life of...
Role of Leadership and Employee Engagement towards Individual Performance of Pharmacy Employees
Employees dissatisfaction to the head of the hospital pharmacy will decrease employees performance and unsatisfied customers. To solve the problems, employees should be based on performance as customer expectations in pr...
A Prospective Study of Adverse Drug Reactions in 1 Month–12 Years Old Pediatric Patients
Adequate controlled clinical trials in pediatric population, especially in oncology and vaccinations are still insufficient due to ethical considerations. Certain conditions in children in general and in Indian children...
Effect of Topical Probiotic on MMP-13 and Collagen III Expression in The Dermis Layer of Male Rats Irradiated with Ultraviolet-B
Nowadays, there is a big interest in the use of topical probiotic preparations for skin health. One of the probiotics therurapeutic benefits is used as anti-aging. During aging, there is stimulation of activator protein-...
Potential of Drug-Drug Interaction in Polypharmacy Prescription: Retrospective Study on a Drugstore in Bandung
Polypharmacy prescription very commonly occurs on prescribing the outpatient and inpatient in every health facility. Polypharmacy may increase the risk of Drug-drug Interactions (DDI’s) which mostly causes harm impact in...