Rapid Detection of Enterohaemorrhagic E.coli Using Phage-Based Bioluminescent Assay
Journal Title: Journal of Clinical and Diagnostic Research - Year 2018, Vol 12, Issue 11
Abstract
ABSTRACT Introduction: There is a need for a reliable, time-saving, and specific detection assay for coliforms, environmental E. coli, and Enterobacteriaceae worldwide. Aim: To innovate a new principle of phage-based rapid diagnostic test for detecting E. coli bacteria in short time and low titer. Materials and Methods: A phage mixture of 200 E. coli specific phages, including 22 specific for Enterohaemorrhagic E.coli (EHEC), were used in a new detection platform, a phage-based Adenylate Kinase Bioluminescence Assay (AKBA). Ten EHEC E. coli and 30 universal E.coli isolates were used for AKBA assay. Results: AKBA showed positive detection of E.coli bacteria at 103 CFU in just 20 minutes. The phage-based detection was highly specific at strain level of E.coli. The sensitivity and specificity of AKBA was 74% and 78%, respectively. Conclusion: A rapid and strain-specific diagnostic test was prepared for E.coli by using coliphages. The significance and impact of the study shows that it might be feasible to formulate a phage-based assay against any Gram negative or positive bacteria using the same approaches of the current AKBA assay with slight modifications.
Authors and Affiliations
Sabah AA Jassim, Rand R Hafidh, Zahraa Q Ali, Ahmed S Abdulamir
Keywords
Related Articles
- EP ID EP524468
- DOI 10.7860/JCDR/2018/36748.12251
- Views 88
- Downloads 0
Histochemical Study of Inflammatory Lesions of Gall Bladder with Reference to Metaplastic Conditions
ABSTRACT Introduction: Cholelithiasis and asymptomatic gallstones may produce pathological changes like hyperplasia and metaplasia of the gall bladder mucosal epithelium. It has been suggested that metaplastic epitheliu...
Neck Circumference and Leg Length as Surrogate Markers of Coronary Artery Disease - Simplifying Cardiac Risk Stratification
Introduction: Coronary angiography is the gold standard for quantification of coronary atherosclerosis. But, being invasive, it has inherent complications. Hence, we examined the accuracy of prediction of coronary angiog...
Comparative Evaluation of Shear Bond Strength of Resin Modified Glass Ionomer Cement and Biodentine as Dentin Substitutes to Bonded Silver Amalgam and Composite Resin
ABSTRACT Introduction: The bond strength between the restorative material and the tooth, as well as between the restorative material and the base, is an important factor and can determine the longevity of a dental treatm...
Single Nucleotide Polymorphisms of XRCC1 and the Risk of Head and Neck Cancer
ABSTRACT Introduction: Head and Neck Cancers (HNCs) accounts for more than 550,000 cases and 380,000 deaths annually, worldwide. Overall, 57.5% of global HNCs occur in Asia especially in India in both sexes. Single Nucle...
Fibroadenoma in Axillary Ectopic Breast Tissue Mimicking Lymphadenopathy
Swellings in the axilla especially in women are always viewed with suspicion owing to a large number of these being associated with breast carcinoma presenting as nodal metastasis. In a country like India, tuberculous ly...