Rapid Detection of Enterohaemorrhagic E.coli Using Phage-Based Bioluminescent Assay
Journal Title: Journal of Clinical and Diagnostic Research - Year 2018, Vol 12, Issue 11
Abstract
ABSTRACT Introduction: There is a need for a reliable, time-saving, and specific detection assay for coliforms, environmental E. coli, and Enterobacteriaceae worldwide. Aim: To innovate a new principle of phage-based rapid diagnostic test for detecting E. coli bacteria in short time and low titer. Materials and Methods: A phage mixture of 200 E. coli specific phages, including 22 specific for Enterohaemorrhagic E.coli (EHEC), were used in a new detection platform, a phage-based Adenylate Kinase Bioluminescence Assay (AKBA). Ten EHEC E. coli and 30 universal E.coli isolates were used for AKBA assay. Results: AKBA showed positive detection of E.coli bacteria at 103 CFU in just 20 minutes. The phage-based detection was highly specific at strain level of E.coli. The sensitivity and specificity of AKBA was 74% and 78%, respectively. Conclusion: A rapid and strain-specific diagnostic test was prepared for E.coli by using coliphages. The significance and impact of the study shows that it might be feasible to formulate a phage-based assay against any Gram negative or positive bacteria using the same approaches of the current AKBA assay with slight modifications.
Authors and Affiliations
Sabah AA Jassim, Rand R Hafidh, Zahraa Q Ali, Ahmed S Abdulamir
Keywords
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- EP ID EP524468
- DOI 10.7860/JCDR/2018/36748.12251
- Views 94
- Downloads 0
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