Rapid detection protocol ofFusarium oxysporum f. sp.cubense race 1 in banana
Journal Title: Biotecnología Vegetal - Year 2021, Vol 21, Issue 2
Abstract
Fusarium wilt of banana is a typical vascular wilt disease caused byFusarium oxysporum f.sp.cubense. The genetic improvement program of the crop needs tools that allow the quickdetection of fungal phytopathogen. The objective of this work was to establishment a simpledetection protocol ofF. oxysporum race 1 inMusa spp. samples by polymerase chain reaction(PCR). The principal related aspects in this protocol are the sampling, fungalin vitro culture,quick DNA extraction method, PCR protocol, electrophoresis separation of PCR products, andgeneral considerations during the work.
Authors and Affiliations
María I. Oloriz, Naylin Machado, Luis E. Rojas, Bárbara Ocaña, Marilín Hernández
Keywords
Related Articles
- EP ID EP712468
- DOI -
- Views 56
- Downloads 0
In vitro establishment of apex and nodule segments of Swietenia macrophylla King
The application of tissue culture techniques in woody plants offers a valuable alternative for the propagation of elite trees. With the objective of obtaining the in vitro establishment of mahogany (Swietenia macrophyll...
Regeneration of plants via somatic embryogenesis in Phaseolus acutifolius A. Gray
Plant regeneration protocols are a requirement to develop plants transformation systems.The capacity of Phaseolus acutifolius A. Gray to regenerate plants from embryogenic callus formation was investigated. Two explants...
Effect of inoculum density and frequency of immersion on the in vitro propagation of Psidium guajava cv. Red dwarf in temporary immersion systems
The objectives of the present work were to study the effect of the inoculation density and the immersion frequency in the multiplication of guava explants in temporary inmersion systems. Systems with flasks of 1.0 liter...
Protocol for Anthurium cubense Engler micropropagation from botanic seed
The popularity of Anthurium cubense, like pot plant, every day it is increased. Likewise, it has begun to extend the line of cultivated products for their employment as foliage. However, the traditional propagation of...
In vitro control of Mycosphaerella fijiensis with isolated bacteria of the banana phyllosphere
Microorganisms with profitable effects to eliminate disseases have been found in the study of microbial communities associated with plants. Little is known about biotic factors in the interaction of Musa-M. fijiensis....