Rapid detection protocol ofFusarium oxysporum f. sp.cubense race 1 in banana
Journal Title: Biotecnología Vegetal - Year 2021, Vol 21, Issue 2
Abstract
Fusarium wilt of banana is a typical vascular wilt disease caused byFusarium oxysporum f.sp.cubense. The genetic improvement program of the crop needs tools that allow the quickdetection of fungal phytopathogen. The objective of this work was to establishment a simpledetection protocol ofF. oxysporum race 1 inMusa spp. samples by polymerase chain reaction(PCR). The principal related aspects in this protocol are the sampling, fungalin vitro culture,quick DNA extraction method, PCR protocol, electrophoresis separation of PCR products, andgeneral considerations during the work.
Authors and Affiliations
María I. Oloriz, Naylin Machado, Luis E. Rojas, Bárbara Ocaña, Marilín Hernández
Keywords
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