RAPID ISOLATION OF THE BIOACTIVE METABOLITE OF CYMBOPOGON PROXIMUS AND DEVELOPMENT OF LC METHOD
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 5
Abstract
Objective: Development of a rapid isolation method to afford the bioactive metabolite (proximadiol: prox) from its herb (Cymbopogan proximus Stapf.) in the purest form and validate an LC method for its determination was our goal in this work. Methods: Prox was isolated by chromatographic techniques from the dichloromethane extract of the herb on alumina column, using petroleum ether-ethyl acetate solvent mixtures with increasing polarity.The determination of prox was achieved by LC method. The chromatographic separation was carried out on Thermo Hypersil ODS (4.5 x 250 mm, 10 µm) column, in the presence of 8-chlorotheophylline as an internal standard. The mobile phase was composed of 0.1 M phosphate buffer (pH: 3.5): methanol (60:40 v/v) and was pumped at a flow rate of 1 ml/min. The detection and quantification were done at 210 nm.Results: The structure elucidation of the isolated compound was identified on the basis of its spectral data.A simple and reproducible LC method was developed for the determination of the isolated purified Prox. Adequate separation and good resolution were obtained between prox and 8-chlorotheophylline. Quantification was achieved at 210 nm over concentration range 12.00-33.60 µg/ml with mean percentage recovery of 99.29±0.340. The method was validated in accordance with USP specifications. All analytical criterions were within acceptable range. The validity of the results was assessed by applying standard addition technique. The results obtained were compared with the reported method.Conclusion: The proposed method could be applied for routine quality control analysis of pharmaceutical formulations containing prox.
Authors and Affiliations
Ola Mohamed El-houssini, Ghada Farouk Metwally
Keywords
Related Articles
- EP ID EP575636
- DOI 10.22159/ijpps.2017v9i5.16954
- Views 45
- Downloads 0
HYDRATE TRANSFORMATION OF SODIUM SULFACETAMIDE AND NEOMYCIN SULPHATE
Objective: Hydrate transformation influence physical properties of the active pharmaceutical ingredients (APIs) such as solubility and stability. This research aimed to analyzed the hydrate transformation of sodium sulfa...
EVALUATION OF TAXIFOLIN AND PHLORETIN AS ANTIANGIOGENIC: AN IN VIVO, IN VITRO EXPERIMENTAL ANALYSIS
Objective: To examine the antiangiogenic effect of flavonoids (taxifolin and phloretin) against various In vivo and In vitro experimental models such as Chick Chorio Allantoic Membrane (CAM) assay, Dorsal Skinfold Chambe...
COMPUTER-AIDED MODEL FOR BREAST CANCER DETECTION IN MAMMOGRAMS
The objective of this research was to introduce a new system for automated detection of breast masses in mammography images. The system will be able to discriminate if the image has a mass or not, as well as benign and m...
EVALUATION OF IMMUNOMODULATORY AND ANTIOXIDANT ACTIVITIES OF POLYSACCHARIDES ISOLATED FROM CALLICARPA MACROPHYLLA VAHL.
Objective: To evaluate the immunomodulatory and antioxidant activities of polysaccharides from Callicarpa macrophylla Vahl. leaves.Methods: Three different fractions of polysaccharides (PC, PH, PA) were isolated. The per...
INDUCTION OF APOPTOSIS BY FATTY ACID RICH FRACTION OF SOLANUM NIGRUM ON CERVICAL CANCER CELL LINES
Objective: Mechanism of cell death inducing pathway of chloroform fraction of methanolic extract of Solanum nigrum were studied in a panel of cervical cancer cell line.Methods: Cells were treated with IC50 doses (previou...