REGULATORY EFFECTS OF MMU-MIR-8114 AND MMU-MIR-3473B IN ALLERGIC MICE INDUCED BY ECHINOCOCCOSIS
Journal Title: Acta Parasitologica et Medica Entomologica Sinica - Year 2024, Vol 31, Issue 3
Abstract
[Objective] This study aimed to explore the regulatory roles of microRNAs (miRNAs) in mice with allergic reactions induced by Echinococcus granulosus. [Methods] A mouse model of Echinococcosis was established, and spleen mononuclear cells were collected for transcriptomic sequencing. The differential expressions of mRNAs and miRNAs between allergic and non-allergic mice were compared. The target regulatory relationships between mRNAs and miRNAs were predicted using TargetScan and miRanda softwares, and core genes were identified through protein-protein interaction (PPI) network analysis. The biological functions of these core genes were further explored via enrichment analysis. Finally, key results were validated using qRT-PCR and Western blot. [Results] A total of 1 642 differentially expressed mRNAs were identified between allergic and non-allergic mice. Among the 18 differentially expressed miRNAs, 60 target genes were identified, with the top 10 genes having the highest connectivity in the PPI network-deemed as core genes. Enrichment analysis showed that these core genes are primarily involved in the NF-kappa B signaling pathway. Based on the negative regulatory effects between miRNAs and mRNAs and the dual-luciferase reporter system, mmu-miR-8114 was found to target Atf3 and mmu-miR-3473b to target Myd88, both of which are related to the NF-kappa B signaling pathway. qRT-PCR validation showed that mmu-miR-8114 and mmu-miR-3473b were downregulated in allergic mice, while Atf3, Myd88, and Socs3 were upregulated. Increased activity of the NF-kappa B signaling pathway was observed in allergic mice through qRT-PCR and Western blot results. [Conclusions] This study reveals the significant role of mmu-miR-8114 and mmu-miR-3473b in regulating mouse allergic reactions through the NF-kappa B signaling pathway, offering new insights into the mechanisms behind allergic reactions caused by Echinococcus granulosus infection.
Authors and Affiliations
Yan MA, Jing HAN, Zhi-yuan FANG, Tailaiti SUBI·, Xiao-dong YU
Keywords
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- EP ID EP753995
- DOI 10-3969/j.issn.1005-0507-2024-03-003
- Views 19
- Downloads 0
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