Relaxant effects of adhatoda vasica on guinea pig Tracheal chains and its possible mechanisms
Journal Title: International Journal of Research in Pharmacology & Pharmacotherapeutics (IJRPP) - Year 2012, Vol 1, Issue 1
Abstract
Adhatoda vasica is a plant believed to have several therapeutic effects, including anti-asthmatic properties. The objective of this study was to investigate the relaxant effects of macerated and soxhlet extracts of leaves of this plant on tracheal chains of Guinea pigs were evaluated. The relaxant effects of 4 cumulative concentrations of macerated and soxhlet extracts (0.25, 0.5, 0.75 and 1.0 W/V) in comparison with saline as negative control and 4 cumulative concentrations of theophylline (0.25, 0.5, 0.75, and 1.0 mM) as positive controls were examined on precontracted tracheal chains of two groups of 6 Guinea pig by 60 mM KCl (group 1, N=6) and 10 µM methacholine the non-incubated tissues (group 2, N = 6) and tissues incubated with 1 µM propranolol (group 3, N = 4)[24]. Decrease in contractile tone of tracheal chains was considered as the relaxant effect. The isolated guinea-pig trachea pre-contracted with KCl, methacholine and tissues incubated with propranolol were used to study the relaxation of macerated and soxhlet extracts of leaves Adhatoda vasica. In the group, 1 experiments only the last two higher concentrations of theophylline and soxhlet extract showed significant relaxant effect compared to that of saline (p<0.001 for both concentrations), which were significantly greater than those of macerated extracts (p<0.001 for all cases). The effects of two higher concentrations of theophylline in this group were significantly greater than those of macerated and soxhlet extracts (P < 0.01). And in group 2 and 3 experiments, both macerated and soxhlet extracts showed concentration-dependent relaxant effects compared to that of saline (p<0.05 to p<0.001 for both extracts). There were no significant differences when comparing the relaxant effects of macerated and soxhlet extracts with those of theophylline in the group 2 experiments. The relaxant effects of macerated, and soxhlet extracts in group 1 were significantly lower than those of groups 2 and 3. These results showed a potent relaxant effect of Adhatoda vasica on tracheal chains of Guinea pigs, which were lower than theophylline at concentrations used.
Authors and Affiliations
Prasanta Kumar Mahapatra
Keywords
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