Resurrection of the Fern "Cheilanthes albomarginata Clarke" Involves Utilization of Trehalose as Energy Source and Accumulation of Protective Stress Metabolites
Journal Title: International Journal of Biochemistry & Physiology - Year 2018, Vol 3, Issue 3
Abstract
Most plants are unable to survive desiccation to an air-dried state, but a small group of plants known as resurrection plants can tolerate extreme desiccation, and regain normal function after rehydration. Cheilanthes albomarginata is one such resurrection fern, which has been analyzed in this study for metabolite changes during resurrection. In this study, we have tested C. albomarginata fronds from ten different geographical regions of Himachal Pradesh for the presence of trehalose, proline, and glycine betaine. The profile of trehalose sugar during resurrection was investigated using chromatographic techniques and enzymatic assays. Analysis of extracted sugars by High performance liquid chromatography (HPLC) showed that trehalose levels were highest in dehydrated stage of C. albomarginata (46 %), which reduced to 28% upon rehydration. Fourier Transform InfraRed spectroscopy (FTIR) analysis of intracellular sugars in crude extracts of C. albomarginata indicated the closest match of the sugar in the samples with trehalose rather than sucrose. Quantitative estimation of extracted trehalose sugar of C. albomarginata using crude trehalase further confirmed the utilization of trehalose during resurrection. Thus, trehalose appears to be the major sugar accumulating during dehydrated condition, with an active role in resurrection of C. albomarginata. Proline and Glycine betaine were observed to accumulate during dehydration stage in C. albomarginata, and remained largely unaltered during rehydrated state, indicating their protective role during resurrection.
Authors and Affiliations
Sourirajan A
Keywords
Related Articles
- EP ID EP396156
- DOI 10.23880/ijbp-16000131
- Views 80
- Downloads 0
Photoadducts and Peroxynitrite: Implications in Pathophysiology and Immunopathology
DNA adducts are a form of DNA damage caused by covalent attachment of a chemical moiety to DNA. Recent evidence suggests associations between the occurrence of adducts formed by specific compounds and various types of to...
Investigation Effect of Free Radical Generated from Cement Dust on Antioxidant Enzymes Activities on Cement Factory Workers
This study investigated effects of cement dust on antioxidant enzymes activities of Obajana Cement factory workers. Sixty (60) workers in different units/sections exposed to cement dust and controls (unexposed to cement...
Current Status of Plant Biochemistry Research
Plant Biochemistry is one of the key areas in Biochemistry that deals with chemical processes and molecular functions of plants. Simply, it helps understanding how biological molecules give rise to the processes that occ...
Resurrection of the Fern "Cheilanthes albomarginata Clarke" Involves Utilization of Trehalose as Energy Source and Accumulation of Protective Stress Metabolites
Most plants are unable to survive desiccation to an air-dried state, but a small group of plants known as resurrection plants can tolerate extreme desiccation, and regain normal function after rehydration. Cheilanthes al...
Evaluation of Anti-Hyperglycaemic Potential of Allium Cepa, Coffee and Oxidative Stress
Allium cepa Linn, botanicals, Liliaceae (family) commonly known as onion, and coffee are special delicacies, and conventional as functional foods that contain physiologically active ingredients that for centuries have be...