Resurrection of the Fern "Cheilanthes albomarginata Clarke" Involves Utilization of Trehalose as Energy Source and Accumulation of Protective Stress Metabolites
Journal Title: International Journal of Biochemistry & Physiology - Year 2018, Vol 3, Issue 3
Abstract
Most plants are unable to survive desiccation to an air-dried state, but a small group of plants known as resurrection plants can tolerate extreme desiccation, and regain normal function after rehydration. Cheilanthes albomarginata is one such resurrection fern, which has been analyzed in this study for metabolite changes during resurrection. In this study, we have tested C. albomarginata fronds from ten different geographical regions of Himachal Pradesh for the presence of trehalose, proline, and glycine betaine. The profile of trehalose sugar during resurrection was investigated using chromatographic techniques and enzymatic assays. Analysis of extracted sugars by High performance liquid chromatography (HPLC) showed that trehalose levels were highest in dehydrated stage of C. albomarginata (46 %), which reduced to 28% upon rehydration. Fourier Transform InfraRed spectroscopy (FTIR) analysis of intracellular sugars in crude extracts of C. albomarginata indicated the closest match of the sugar in the samples with trehalose rather than sucrose. Quantitative estimation of extracted trehalose sugar of C. albomarginata using crude trehalase further confirmed the utilization of trehalose during resurrection. Thus, trehalose appears to be the major sugar accumulating during dehydrated condition, with an active role in resurrection of C. albomarginata. Proline and Glycine betaine were observed to accumulate during dehydration stage in C. albomarginata, and remained largely unaltered during rehydrated state, indicating their protective role during resurrection.
Authors and Affiliations
Sourirajan A
Keywords
Related Articles
- EP ID EP396156
- DOI 10.23880/ijbp-16000131
- Views 60
- Downloads 0
Introducing the Evolutionary Cell Memory (ECM) Hypothesis
This research study has gone through more than 34 sample tumors in Violet Cancer Institute (VCI) to find the cancer cells’ resemblances to the primitive eukaryote cells in 3.5 billion years ago before the entrance of the...
Biochemical Characterization of Two Major Proteins of the Hamster Sperm Acrosomal Matrix
An acrosomal fraction, termed the ALM (acrosomal lamina complex) was isolated from hamster cauda epididymal spermatozoa that contain specific domains of the acrosomal matrix and an adherent detergent-insoluble complex, t...
Altered Cell Survival Signaling in the Cerebral Cortex as a Long Term Effect of Neonatal Hypoglycemia
Neonatal hypoglycemia can cause drastic alterations in brain function by damaging areas like cortex, hypothalamus and cerebellum. We evaluated the extend of cell damage caused by severe neonatal hypoglycemia by TOPRO- 3...
Isolation, Production and Optimization of Cellulase from a Combination of Aspergillus Niger and Trichoderma Viride Isolated from Decaying Woods
Cellulase is an enzyme that is capable of hydrolyzing the complex organic form of cellulose into simple sugar (glucose). Cellulase production by a combination of Aspergillus niger and Trichoderma viride isolated from dec...
Serum lipid Profile and Nutritional Status of the Hypertensive Outpatients in University College Hospital, Ibadan
Background: Dyslipidaemia among hypertensive patients increases risks of cardiovascular morbidity and mortality. Nutritional status of the patients relates to the clinical diagnosis in confirming the cause of their ailme...