Role of oxidative stress in ethanol induced germ cell apoptosis - An experimental study in rats
Journal Title: Indian Journal of Clinical Biochemistry - Year 2005, Vol 20, Issue 2
Abstract
The study was undertaken to evaluate the possible involvement of oxidative stress in the pathogenesis of ethanol induced testicular atrophy in rats. Adult male rats were orally administered ethanol at a dose of 1.6g /kg body weight/ day for four weeks. Twenty four hours after the last treatment the rats were sacrificed using anesthetic ether. Testes were removed and weighed. Apoptosis was studied by using the Feulgen reaction on 5μ thin paraffin sections of testis. Testicular homogenate was prepared and centrifuged. The supernatant was used for the estimation of extent of lipid peroxidation and antioxidant defense status. There was significant reduction in body weight; and in testicular weight and diameter in ethanol treated rats. Extent of germ cell apoptosis was significantly high in ethanol treated rats. Ethanol treated rats showed significantly high tissue TBARS level and glutathione S-transferase activity; and low tissue ascorbic acid, reduced glutathione, superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase activities. Chronic ethanol administration resulted in high oxidative stress in the testes either due to increased extent of lipid peroxidation or due to decreased antioxidant defenses, and thereby induces germ cell apoptosis leading to testicular atrophy.
Authors and Affiliations
M Maneesh, H Jayalekshmi, Sanjiba Dutta, A Chakrabarti, D Vasudevan
Keywords
Related Articles
- EP ID EP91185
- DOI -
- Views 115
- Downloads 0
DETECTION OF ENZYMES DEHYDROGENASES AND PROTEASES IN BRUGIA MALAYI FILARIAL PARASITES
Lymphatic filariasis caused mainly by infection from W. bancrofti and B. malayi remains a major cause of clinical morbidity in tropical and subtropical countries. Analysis of B.malayi mf, infective larval and adult wor...
EFFECT OF ANTIOXIDANTS AND ANTIBIOTICS ON LEVELS OF SEMINAL OXIDATIVE STRESS IN LEUKOCYTOSPERMIC INFERTILE MEN
Defective sperm function is the most common cause of infertility. A prospective study was carried out to correlate the concentration of nitrite (the stable metabolite of nitric oxide) in seminal plasma with leukocytosp...
EFFECT OF OCIMUM SANCTUM (TULSI) AND VITAMIN E ON BIOCHEMICAL PARAMETERS AND RETINOPATHY IN STREPTOZOTOCIN INDUCED DIABETIC RATS
This study was carried out to see the effect of the aqueous extract of Ocimum sanctum Linn (Tulsi) with Vitamin E on biochemical parameters and retinopathy in the streptozotocin-induced diabetic albino male rats. Adult...
Isolation and Culture of Leydig Cells from Adult Rats
Role of reactive oxygen species and antioxidants on pathophysiology of male reproduction
The excessive generation of reactive oxygen species (ROS) by abnormal spermatozoa and contaminating leukocytes has been defined as one of the few etiologies for male infertility. Administration of antioxidants in patient...