Role of S-100 immunostain as a diagnostic aid to demonstrate nerve changes in lepromatous leprosy and borderline lepromatous leprosy
Journal Title: Medpulse International Journal of Pathology - Year 2018, Vol 8, Issue 1
Abstract
Background: Leprosy is a chronic infectious disease, which has been targeted for eradication in India. To achieve complete elimination, it is necessary to achieve early and definitive diagnosis. A definitive diagnosis of Leprosy requires demonstration of either Acid Fast Bacilli & nerve damage. There is considerable difficulty in diagnosis of early lesions of leprosy. S-100 immunostain helps in demonstration of nerve damage and thus helps in earlier diagnosis. Although few studies have been done to demonstrate nerve changes in Tuberculoid spectrum of leprosy using S-100, ours is the first study to demonstrate the nerve changes seen in Lepromatous spectrum of leprosy. This may help to provide new diagnostic aids in the diagnosis of Lepromatous spectrum Objectives: 1.To study the neuronal changes in Lepromatous spectrum of Leprosy 2.To demonstrate the various patterns of neuronal staining in Lepromatous spectrum of Leprosy, using S-100 immunostaining Methodology: Skin biopsies of twenty four cases of histopathologically confirmed cases of leprosy belonging to Lepromatous spectrum were subjected to S-100 immunoperoxidase stain Results: In our study, on S-100 immunostaining there were four patterns of nerve staining across the Lepromatous spectrum, namely absent, fragmented, infiltrated & intact. Of this absent pattern of immunostaining was the most common pattern Conclusion: For demonstration of nerve changes, S-100 is superior to H&E stain. A few studies have been done to demonstrate nerve changes in Tuberculoid spectrum. Ours is the first study to apply S-100 immunostain to demonstrate the nerve changes in Lepromatous spectrum of Leprosy. We found that absent pattern of staining predominated in Lepromatous spectrum.
Authors and Affiliations
Anand Mohanraj
Keywords
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- EP ID EP457141
- DOI 10.26611/105815
- Views 99
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