Root Cause Analysis of Insufficient Centrifugation and Sample Aliquoting of Blood Samples in Clinical Chemistry Laboratory
Journal Title: Scholars International Journal of Biochemistry - Year 2018, Vol 1, Issue 2
Abstract
The study aims to find out root cause for insufficient centrifugation and sample aliquoting of blood samples in clinical chemistry laboratory. Blood samples centrifuged in the laboratory were aliquoted by technicians in to 1.5 ml eppindorf cups for placement in to automated chemistry analyser. The aliquotes were allowed to settle for 1 hour and observed for presence of red rim of settled RBC at the bottom of the cups, indicating insufficient centrifugation and sample aliquoting. Frequency of such samples were analysed for time of day, type of sample tube, amount of residual serum/plasma after aliquoting, origin of sample from hospital departments. Total 13491 sample tubes analysed in 20 days at Biochemistry laboratory of New civil hospital Surat. There were 248 samples showing red rim of RBC at the bottom of the cups. Out of 248, samples received at night constituted 78%. Serum sample have 3 times chances of having insufficient centrifugation and sample aliquoting as compared to fluoride plasma. 86% of sample had residual serum/plasma of <200 ul. Neonate sample have 7 times chances of having insufficient centrifugation and sample aliquoting as compared to adult samples. 97% of samples having insufficient centrifugation and sample aliquoting were received from wards while only 3% were received from OPD. Under filling of collection tubes, neonatal samples, samples collected in plain vacuum tubes, samples collected by phlebotomist on duty at night are root causes for insufficient centrifugation and sample aliquoting.
Authors and Affiliations
Dr. Khushbu Soni, Dr. Riddhi Patel
Keywords
Related Articles
- EP ID EP543258
- DOI -
- Views 55
- Downloads 0
Cholesterol Fasting or Non Fasting? Patient Variables in Cholesterol Testing Values
Fasting for at least eight hours prior to a lipid test has been standard practice in India and internationally for many years. However, a growing body of evidence and international expert opinion suggests that a non-fast...
Relationship between Serum Levels of Albumin and C - reactive protein (CRP) In Patients with Chronic Kidney Disease
Protein- energy malnutrition, wasting and inflammation are frequent complication among patient with Chronic kidney disease (CKD). Malnutrition is associated with cardiac co-morbidity, inflammation and poor survival in CK...
Fast, Accurate and Cost-Effective Detection and Diagnostics of Diabetes Mellitus Thanks to Using New, Patented Diagnostic Procedure and Clinical Expert Decision Support System Bio Analyst
The main aim of this study is to present a new, effective approach to diagnose Diabetes Mellitus in a timely, accurate and cost-effective manner, thanks to using technology and new, patented diagnosing procedure. This st...
Spectroscopic Characterization of Phyllanthus muellerianus Plant Leaves Dye
The aim of this investigation is to characterize the Phyllanthus muellerianus plant dye used as a colour source in painting walls and craft works for ages in Oghe, Ezeagu Local Government Area of Enugu State. Spectroscop...
Serum Cystatin C and Creatinine level among Chronic Kidney Disease Patients Undergoing Hemodialysis
Chronic kidney disease (CKD) is one among the slowly progressive diseases of kidney function characterized generally by low glomerular filtration rate (GFR). The replacement therapy of renal failure by hemodialysis inv...