RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF GABAPENTIN AND METHYLCOBALAMIN IN TABLET DOSAGE FORMS
Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 7
Abstract
A simple, accurate, economical and reproducible HPLC method for simultaneous estimation of two component drug mixture of Gabapentin and Methylcobalamin (MCB) in combined tablet form have been developed. The detection was performed at 271 nm. The retention time of Gabapentin and Methylcobalamin was found to be 2.5 min and 3.08 min respectively. Linearity was observed in concentration range of 600-1800mcg/ml of Gabapentin and 1-3mcg/ml of Methylcobalamin. The reverse phase chromatographic method used C18 column and 0.1% Orthophosporic acid:acetonitrile in ratio of 55:45 as mobile phase. Results of analysis were validated statistically and by recovery studies. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision, and robustness.
Authors and Affiliations
Shashe Kumar P*, , Ramamohan Reddy T, , Umamaheshwara Rao V
Keywords
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