RP – HPLC Method Development and Validation for Simultaneous Estimation of Irbesartan, Amlodipine and Hydrochlorothiazide in Bulk and Tablet Dosage Forms
Journal Title: Journal of Pharmaceutical and Medicinal Chemistry - Year 2017, Vol 3, Issue 2
Abstract
A reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Irbesartan, Amlodipine besylate and hydrochlorothiazide in bulk and Pharmaceutical formulation using RPC18 Column. The mobile phase (Acetonitrile: methanol: 50mM phosphate buffer adjusted to pH 3.2 with orthophosphoric acid) was pumped at a flow rate of 1.2 mL/min in the ratio of 20:50:30% v/v and the eluent was monitored at 250 nm. Linearity was obtained in the concentration range of 5 30 µg/ml for Amlodipine Besylate, 20 120 µg/ml for Irbesartan and 10 60 µg/ml for Hydrochlorothiazide. The method was statistically validated and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed HPLC method. Due to its simplicity, rapidness, high precision and accuracy, the proposed HPLC method can be applied for determining Irbesartan, Amlodipine and hydrochlorothiazide in bulk and in pharmaceutical dosage form.
Authors and Affiliations
Gandla Kumaraswamy
Keywords
Related Articles
- EP ID EP469496
- DOI 10.21088/jpmc.2395.6615.3217.4
- Views 129
- Downloads 0
Effect of Antibiotics, Rehydration Therapy, Zinc and Probiotics in Diarrhoea in Children below 5 Years of Age
Introduction: Acute diarrhoea is defined as an abnormally frequent discharge of semisolid or fluid faecal matter from bowel, lasting less than 14 days. Enteropathogens that cause diarrhoea may affect physiology of gut in...
Effect of Microwaving and Some Cooking Methods on Enrofloxacin in Chicken Meat
The present study was undertaken to determine the effects of microwaving and some cooking methods like boiling and deep-frying on Enrofloxacin (ENR) in chicken meat. Chicken meat samples incurred with known concentration...
RP – HPLC Method Development and Validation for Simultaneous Estimation of Irbesartan, Amlodipine and Hydrochlorothiazide in Bulk and Tablet Dosage Forms
A reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Irbesartan, Amlodipine besylate and hydrochlorothiazide in bulk and Pharmaceutical formulation using RP...
Bioinorganic Chemistry of Metal Chitosans and Nucleic Acids Complexes
Bacterial samples were collected from consecutive diabetic patients with foot wounds at the Vascular Surgery Unit and the Diabetic Foot Unit of Alexandria Main University Hospital, Faculty of Medicine, Alexandria Univers...
Analytical Method for the Simultaneous Estimation of Sitagliptin and Simvastatin using RP-HPLC
A novel, rapid, precise and accurate high performance liquid chromatographic method was developed and validated for the simultaneous determination of Sitagliptin phosphate and Simvastatin in bulk drug and pharmaceutical...