RP-HPLC Method Development and Validation for Simultaneous Estimation of Linezolid and Cefixime in API and Pharmaceutical Dosage Form M Naga,
Journal Title: International Journal of Pharma Research and Health Sciences - Year 2017, Vol 5, Issue 4
Abstract
A new sensitive, accurate, precise and validated RP-HPLC method was developed for the concurrent assessment of linezolid and cefiximein in API and pharmaceutical dosage form. The chromatographic separation was achieved on WATERS Alliance 2695: Empower 2, 996 PDA detector, Phenomenex Luna C18 column, mobile phase consist of a mixtures of methanol: water pH 3.8 (47:53%v/v) with detection wavelength at 260nm. The retention times for linezolid and cefixime were found to be as 2.4 and 3.9min respectively. The developed method was validated for the various parameters as per ICH (Q2R(1)) guidelines. The system suitability, specificity, accuracy, precession, detection and quantification limits and robustness studies showed the developed method was found to within the limits. Hence the developed method can be used for the routine analysis of linezolid and cefiximein in bulk and pharmaceutical dosage forms.
Authors and Affiliations
Keywords
Related Articles
- EP ID EP309659
- DOI -
- Views 93
- Downloads 0
Assessment of Prescription Pattern and Monitoring Adverse Drug Reactions of Corticosteroid Usage in a Teaching Hospital
Objective: To analyze the prescription pattern and to monitor the adverse drug reactions due to corticosteroid usage Materials and Methods: A prospective observational study was conducted at the medicine and pediatric in...
Formulation and Evaluation of Domperidone Fast Dissolving Film by Using Different Polymers
The present study deals with the investigation carried out on developing a fast dissolving delivery system releasing Domperidone concomitantly in stomach for treating vomiting and motion sickness. Domperidone FDOF was pr...
Phytotherapeutic drugs used by the tribal folk of Achanakmar Amarkantak Biosphere Reserve, Central India
The Ethnomedicinal information of Baiga tribes of Achanakmar Amarkantak Biosphere Reserve (AABR), Bilaspur, Madhya Pradesh and Chhattisgarh, India was collected through interviews and discussion regarding the plant presc...
Evaluation of Anxiolytic, Antidepressant and Anti- Inflammatory Activities of Ethanolic Extract of Urena lobata Leaf
Depression is one of the major mental disorders. It affects up to 25% of women and 12% of men and is a highly chronic disorder. Antidepressant drugs used in the treatment of major depressive disorders are believed to act...
Ethnopharmacological survey on bone healing plants with special references to Pholidota articulata and Coelogyne cristata (Orchidaceae) used in folk tradition of Kumaon, Uttarakhand, India
Aim: This study was aimed to conduct an ethnopharmacological survey and document the medicinal plant used for healing fractures in folk tradition of Kumaon, Uttarakhand, India. Materials and methods: The Ethnopharmacolog...