RP-HPLC Method Development and Validation for Simultaneous Estimation of Linezolid and Cefixime in API and Pharmaceutical Dosage Form M Naga,
Journal Title: International Journal of Pharma Research and Health Sciences - Year 2017, Vol 5, Issue 4
Abstract
A new sensitive, accurate, precise and validated RP-HPLC method was developed for the concurrent assessment of linezolid and cefiximein in API and pharmaceutical dosage form. The chromatographic separation was achieved on WATERS Alliance 2695: Empower 2, 996 PDA detector, Phenomenex Luna C18 column, mobile phase consist of a mixtures of methanol: water pH 3.8 (47:53%v/v) with detection wavelength at 260nm. The retention times for linezolid and cefixime were found to be as 2.4 and 3.9min respectively. The developed method was validated for the various parameters as per ICH (Q2R(1)) guidelines. The system suitability, specificity, accuracy, precession, detection and quantification limits and robustness studies showed the developed method was found to within the limits. Hence the developed method can be used for the routine analysis of linezolid and cefiximein in bulk and pharmaceutical dosage forms.
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