RP-HPLC Method for Quantification of Doxorubicin in Presence of Folic Acid
Journal Title: International Journal of Pharma Research and Health Sciences - Year 2017, Vol 5, Issue 5
Abstract
Colloidal delivery systems of doxorubicin (Dox) have been recently gained much importance for tumor targeted delivery. Many of the reported systems present Dox in delivery systems comprising of folic acid (FA). Herein we aimed for a reversed-phase high performance liquid chromatography (RP-HPLC) method for the quantification of Dox in presence of FA. The method employed a mixture of acetonitrile and water (containing 0.2% v/v formic acid) in the ratio 1:1 (v/v) as the mobile phase. It was observed that employing a detection wavelength of 480 nm eliminates any possible interference from FA. The developed method could be applied for quantification of doxorubicin during assay, drug loading and drug release studies where there are chances of folic acid interference.
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