RP-HPLC method for simultaneous estimation of Candesartan and Amlodipine in bulk and pharmaceutical dosage forms
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2014, Vol 2, Issue 4
Abstract
A simple, rapid, accurate and precise isocratic reversed phase high performance liquid chromatographic method has been developed and validated for simultaneous estimation of Candesartan and Amlodipine in tablet dosage forms. The chromatographic separation was carried out on C18 analytical column (150 mm x 4.6 mm I.D., 5 µm particle size) with a mixture of phosphate buffer pH 6.8 was adjusted with potassium hydroxide and acetonitrile in the ratio of 35:65 V/V as a mobile phase at a flow rate of 1.0 mL/min. UV detection was performed at 238 nm. The retention times were 3.610 minutes and 4.773 minutes for Candesartan and Amlodipine respectively. Calibration plots were linear (r2=0.999 for Candesartan and 1 for Amlodipine) over the concentration range of 4-24 µg/mL for Candesartan and 2.5-15 µg/mL for Amlodipine. The method was validated for linearity, precision, accuracy, ruggedness and robustness. The proposed method was successfully used for simultaneous estimation of Candesartan and Amlodipine in tablet dosage forms. Validation studies revealed that method is specific, rapid, reliable and reproducible. The high % recovery and low % RSD confirms the suitability of the proposed method for routine quality control analysis of Candesartan and Amlodipine in bulk and tablet dosage forms.
Authors and Affiliations
Raja B, Lakshmana Rao A
Formulation and in-vitro evaluation of Levocetirizine dihydrochloride and Montelukast sodium bilayered tablet for bi-modal drug release
The present study aims to formulate and evaluate Levocetirizine hydrochloride and Montelukast sodium bilayered tablet for treating nasal allergic rhinitis effectively. By combining Levocetirizine with Montelukast gives...
Formulation and evaluation of floating bilayer tablets of propranolol hydrochloride
Propranolol hydrochloride bi-layered, gastro-retentive floating tablets were formulated to increase the residence time of the drug by direct compression technique. The first layer of sustained layer was punched and sec...
A new method development and validation for the simultaneous estimation of Zolpidium and Melatonin by using RP HPLC in a bulk and pharmaceutical dosage forms
A simple and selective Liquid Chromatography method was described for the determination of Melatonin and Zolpidem tartarate in tablet dosage forms. Chromatographic separation was achieved on a c18 column using mobile p...
Effect of casting solvent on permeability of Anastrazole through Eudragit RL100 films
In the present work, Eudragit RL100 films were prepared by using various casting solvents such as Acetone, chloroform, dichloromethane and ethyl acetate. The influence of solvent on the diffusion characteristics of dru...
In vitro cytotoxic studies of various formulations of Indian medicinal plants
The present study explores the cytotoxic activity of various polyherbal formulations prepared from the combination of chloroform extracts of leaves of Tevetia peruviana, Waltheria indica and Toddalia asiatica. Polyherb...