RP-UFLC-DAD METHOD FOR THE DETERMINATION OF PIPERACILLIN AND TAZOBACTAM SODIUM IN BULK AND MARKETED FORMULATIONS
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2016, Vol 5, Issue 1
Abstract
Aim: To develop a simple, sensitive, rapid, robust and reproducible method for the simultaneous determination of piperacillin and tazobactam sodium using ultra fast chromatographic method (UFLC). Materials and Methods: The analysis was performed on Phenomenex C18 column using acetonitrile, methanol and 1% orthophosphoric acid (pH 2.5) in the ratio of 50:30:20 v/v/v as mobile phase with flow a rate 1ml/min. The eluents were monitored with PDA detector at 225nm. The method was validated as per Q2 (R1) guidelines. The proposed optimised method is having linearity in the concentration range from 5-25 μg/ml of tazobactam sodium and 40-200μg/ml for piperacillin. Results: In this developed method piperacillin and tazobactam sodium were eluted at a retention time of 4.5 ± 0.1min and 3.2 ± 0.1min respectively. The present method is linearity, accurate, precise and has passed all system suitability parameters. Conclusion: The proposed method can be readily utilized for determination of piperacillin and tazobactam sodium.
Authors and Affiliations
Kolli Anupama| Pharmaceutical Analysis Group, Department of Pharmaceutical Chemistry, JSS College of Pharmacy, JSS University, Mysuru – 570015, Karnataka, India, B M Gurupadayya*| Pharmaceutical Analysis Group, Department of Pharmaceutical Chemistry, JSS College of Pharmacy, JSS University, Mysuru – 570015, Karnataka, India, T Sireesha| Pharmaceutical Analysis Group, Department of Pharmaceutical Chemistry, JSS College of Pharmacy, JSS University, Mysuru – 570015, Karnataka, India, R Sairam Aditya| Pharmaceutical Analysis Group, Department of Pharmaceutical Chemistry, JSS College of Pharmacy, JSS University, Mysuru – 570015, Karnataka, India
Keywords
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