Scaffold Free 3D Culture of Mesenchymal Stem Cells; Implications for Regenerative Medicine
Journal Title: Journal of Transplantation & Stem Cell Biology - Year 2015, Vol 2, Issue 1
Abstract
Mesenchymal stem cells (MSCs) are recognized as important sources for regenerative medicine (RM). Adherent cultures, long time considered definitory for MSCs phenotype, are relatively easy to perform and to assess. Two dimensional cell expansion systems, however, have been shown to decrease stemness and to induce cellular senescence therefore impeding on MSC therapeutic potential. Multicellular aggregates known as spheroids are a form of three dimensional (3D) culture increasingly recognized to improve MSC differentiation potential to mesenchymal and non-mesenchymal lineages, to increase cytokine and growth factor release as well as cell trafficking and survival after transplantation. Data about improved vasculogenesis and tissue regeneration potential of spheroid cultured MSCs are already available, making this method attractive for regenerative strategies in wound healing, revascularization after myocardial and cerebral infarctation or peripheral ischemia. In-depth genetic and epigenetic profiling of MSCs in this particular state would be needed in order to predict their therapeutic potential for RM. Better understanding of changes in cellular phenotype that occurs in spontaneous 3D and the implication for in vivo cell behavior as well as cell host interaction after transplantation are required before clinical applications.
Authors and Affiliations
Luminita Simion Labusca
Keywords
Related Articles
- EP ID EP207559
- DOI 10.13188/2374-9326.1000008
- Views 122
- Downloads 0
Stem Cell Therapies in Regenerative Medicine and Diabetes Mellitus: Advances, Constraints and Future Prospects
Stem cell therapy is one of the most promising treatments for the near future. This kind of therapy can ameliorate or even reverse some diseases and have potential applications in regenerative and replacement medicines a...
Cognitive Changes after Cellular Therapy in a Case of Intellectual Disability
Intellectual Disability (ID) is a developmental disability characterized by sub average intellectual functioning, occurring in 2-3% of the population. The underlying cause in ID is the sub-functioning of the neurons whic...
Regulation of Hypoxia-Induced Cell Death and Application of Hypoxic Preconditioning to Stem Cell Transplantation
Hypoxia is a commonly encountered feature of the cellular microenvironment in a number of processes in which programmed cell death (apoptosis) affects disease progression. These diseases include myocardial infarction, st...
Generation of Neural Cells by Direct Cellular Reprogramming
Cellular reprogramming is a new technique that is currently explored for its potential to manufacture human cell types in vitro. Direct cellular reprogramming allows the direct conversion of cells into a target cell type...
Organ Bioengineering, Regeneration and Repair: Where the Future of Organ Transplantation is Taking Shape
Although organ transplantation is now widely viewed as one of the monumental achievements in the history of modern medicine, that was not always the case. Indeed, back at the beginning of the 20th century the idea of rep...