SDS-PAGE Band Patterns of Partially Purified Peroxidase Isozymes Extracted from Telfairia Mosaic Virus (TeMV) Infected and HealthyP lant Leaves of Telfairia occide ntalis (Hook F.)
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 1
Abstract
This study was carried out to separate Telfairia occidentalis peroxidase isozymes extracted from leaves of Telfairia mosaic virus (TeMV) infected and healthy plants by SDS-PAGE and compare their banding patterns for difference. Telfairia occidentalis plants were grown under green house condition and arranged in a completely randomized design prior to inoculation. Randomly selected plants were inoculated with TeMV. Peroxidase isozymes extracted from healthy and infected plants were separated by SDS-PAGE, using 12.5% resolving gel buffered at pH 8.8 and 5% stacking gel in tris glycine buffered at pH 6.8. The isozymes profiles of healthy and infected plants were qualitatively analyzed for presence or absence of bands and quantitatively using Rf values. Analysis of the peroxidase isozyme profiles revealed that both healthy and infected T. occidentalis plants have seven identical bands each with Rf values 0.09, 0.30, 0.35, 0.43, 0.55, 0.68, 0.83 and molecular weights of 66.07, 36.31, 30.20, 23.44, 16.60, 11.75 and 7.08 respectively. The results suggest that SDS-PAGE of peroxidase enzymes cannot be used for detection of TeMV infection of T. occidentalis.
Authors and Affiliations
Mofunanya, A. A. J, Egbe, A. O.
Keywords
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- EP ID EP393362
- DOI 10.9790/3008-1301037277
- Views 81
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