Seed color assessment in rapeseed seeds using Color and Near Infrared Reflectance Spectrometers
Journal Title: Rośliny Oleiste - Oilseed Crops - Year 2009, Vol 30, Issue 1
Abstract
One of the basic directions in the breeding of new varieties of rapeseed is development of yellow seeded rapeseed. The advantage of yellow seeds is reduced amount of polyphenols and fiber in seeds. Yellow-seeded rapeseed contains significantly more oil and protein and less of total dietary fiber than black-seed. Visual classification method of seed color has been developed and applied in breeding works conducted in IHAR. Such examination is very unreliable and depends strongly on experience of a person performing tests, external light circumstances and other parameters, like physiological status of seeds. The Hunter Labs spectrocolorimeter (Colorflex) was applied to minimize the subjectivity of color estimations.Transfer of visual assessment results into Colorflex readings gives satisfactory accuracy. The regression equation error is 0.4 what comprises the 0.5 minimal visually estimated differences. Calibration was done also for NIRS machine using different spectrum ranges. The calibrations using NIRS were in good agreement with Colorflex measurements and can be used as replacement of the Colorflex method. The most reliable estimations were obtained with calibration covering full spectrum (VIS +NIR). The calibration in VIS range was slightly worse. Calibration in only NIR range was giving significantly higher error, but still acceptable, what allows to apply also popular NIR spectrophotometers covering only NIR range.Color estimation with NIRS can be added to the existing calibrations used to measure other seed components (glucosinolate, fat, protein, fiber, etc) so more complex seed examination can be achieved.Repeatability of results is very good for Colorflex readings and acceptable for NIRS measurements. Both methods can be used for seed color assessment for breeding purposes.
Authors and Affiliations
Krzysztof Michalski
Keywords
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