Separation of betalains by gradient high-speed counter-current chromatography
Journal Title: Challenges of Modern Technology - Year 2016, Vol 7, Issue 1
Abstract
A study on separation of betalain mixture obtained from red beet juice (Beta vulgaris L.) by analytical high-speed counter-current chromatography (HSCCC) was performed. The extract was obtained by thermal treatment of acidified red beet juice for 30 min in 85 °C. The pigment mixture consisted of betanin/isobetanin as well as their decarboxy- and dehydro-derivatives. The HSCCC process was accomplished in the ‘tail to head’ mode with two polar solvent systems containing salt: BuOH-EtOH-NaClsolution-H2OH3PO4 (1300:700-1000:1300:700:2.5-5.5 (system I), 1300:200-400:1300:700:2.5-4.5 (system II); v/v/v/v/v). The retention of the stationary phase was 73% (system I) and 79% (system II). The mobile phase was pumped at 2 ml/min flow rate. HPLC-DAD-ESI-MS analyses were performed in reversed phase mode for the obtained HSCCC fractions and crude extract. The solvent systems enabled separation of betanin and decarboxy-betanins (system I and II) as well as neobetanin (system II). Additionally, some pure fractions of 17-decarboxy-betanin and 2,17-bidecarboxy-betanin were obtained in system II.
Authors and Affiliations
Aneta Spórna-Kucab, Dominika Udziela, Monika Sobocińska, Sławomir Wybraniec
Keywords
Related Articles
- EP ID EP199181
- DOI -
- Views 96
- Downloads 0
Effect of plasticizers, superplasticizers and silica fly ash on concrete water-resistance
Concrete is one of the basic construction materials. It is a composite made of cement, water, coarse and fine aggregate. In the past, concrete was produced only with primary components, and it was often of poor quality....
The framework model of the social participation in the process of the local spatial planning
Different forms of planning and urban design works are used in the process of decision-making on the local level, starting with visions of development, through comprehensive or master plans, finishing with the implemente...
Research on solvent systems with tetra-n-butylammonium bromide for counter-current chromatography of betalains
In this study, new two-phase solvent systems for counter-current chromatography (CCC) consisting of n-butanol and water as well as various amounts of acetic acid, acetonitrile, ethanol, acetone or ethyl acetate were test...
Research on dehydrogenation of gomphrenin in heated ammonia solutions of organic solvents
In this report, the influence of ammonia on dehydrogenation of gomphrenin pigment, isolated from purple inflorescences of Gomphrena globosa L., in selected organic solvents (methanol, ethanol, acetone and acetonitrile) a...
Temperature effect on explosion parameters of hydrogen-air deflagrations in presence of water vapor
Results of investigation of hydrogen-air deflagrations phenomenon in closed vessel in various initial temperatures and volume fraction of water vapor are presented in following paper. Tests were performed in apparatus wh...