Serological and genetic analysis of a Bel subtype caused by a novel 29insG mutation: a case report
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 10
Abstract
Objective To investigate the serological characteristics and molecular mechanism of a case of Bel subtype. Methods Serological method was used to analyze the ABO blood group of the patient, absorption and elution test was used to detect the weak antigen on red blood cells, and molecular biology method was used to analyze the ABO gene sequence of the patient. Results The forward typing of the patient was O type, but the reverse typing was B type. The weak B antigen was confirmed by absorption and elution method. This mutation was not found in ISBT and related literature, and was discovered for the first time. The patient's blood type was Bel (29insertG)/O.01.01 based on the sequence of exon 3-7. Conclusion In the case of ABO blood group incompatibility, the combination of serological and molecular biological detection techniques can improve the accuracy and reliability of ABO blood group identification.
Authors and Affiliations
Renlong ZHOU, Cuiyun ZHANG, Guoqing HUANG
Keywords
Related Articles
- EP ID EP749850
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.10.021
- Views 15
- Downloads 0
Syphilis screening results of blood donors in Yinchuan from 2018 to 2021
Objective To analyze the syphilis(TP) screening results of blood donors in Yinchuan from 2018 to 2021, and to provide scientific basis for assessing the risk of syphilis infection among the blood donors. Methods Retrospe...
Influence of blood donation service on the autonomous motivation of blood donors
Objective To explore the influence of blood donors′ demographic characteristics and blood donation service experience on voluntary blood donation motivation. Methods Using self-made questionnaire, stratified sampling met...
Establishment and capacity analysis of the platelet donor database with HPA and HLA genotype in Nanjing
Objective To establish a known HPA/HLA genotype platelet donor database in Nanjing, analyze the polymorphism of HPA-1~6w, -15 and HLA-A, -B and evaluate the matching probability and appropriate capacity of the database f...
Analysis of guiding value and effect of TEG in clinical application of cryoprecipitation
Objective To retrospectively analyze the guiding significance and effect of thromboelogram (TEG) in the clinical use of cryoprecipitation. Methods A total of 289 patients with fibrinogen reduction, admitted to our hospit...
Study on RHD and RHCE gene mRNA based on three generation sequencing technology
Objective To establish RH gene mRNA sequencing method based on nanopores sequencing and to explore the RHD and RHCE mRNA transcripts in D positive and Del individuals. Methods From March 2021 to May 2022, 5 RhD positive...