Serological Evaluation of Early Cases of Japanese Encephalitis by ELISA In Patients at a Tertiary Care Teaching Hospital
Journal Title: International Journal of Medical Research Professionals - Year 2017, Vol 3, Issue 4
Abstract
Introduction: Japanese encephalitis virus (JEV) is the most common cause of viral encephalitis in the world, causing an estimated 45,000 cases and 10,000 deaths annually. Even with the best laboratory facilities, JEV cannot usually be isolated from clinical specimens, probably because of low circulating viral numbers and the rapid development of neutralizing antibodies. The diagnosis is therefore usually made serologically. Hence, present study was undertaken to serologically evaluate early cases of J. encephalitis by ELISA in patients reporting to Patna medical college; a tertiary care teaching hospital. Material and Methods: The present study was carried among 304 cases, reporting to Patna medical college, Patna and samples reaching from different districts of Bihar were included in the study. Samples were collected and transported to department of microbiology Patna Medical College for the IgM against Japanese encephalitis detection through ELISA. Results: A total 304 serum samples were tested for JE antibody from suspected encephalitis patient of Bihar state area. The persons with acute history of fever, headache, corrhyza or flue like acute illness for last few days prior to collection of samples. Out of 304 selected case 65 (21.38%) showed antibody against JE virus. Through JE viral activity was seen in all age groups yet the activity was more prominent among the children age groups. Both male and female had the virus activity with equal frequency (p> 0.05). Conclusion: JE test by ELISA i.e, IgM capture ELISA is highly sensitive and confirmatory and can differentiate, infection type, intensity and presence of JE viral strain, detect specific IgM in CSF or in the blood.
Authors and Affiliations
Ravi Kumar, Krishna Kumar Mani
Keywords
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- EP ID EP546573
- DOI 10.21276/ijmrp.2017.3.4.006
- Views 72
- Downloads 0
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