Short-term storage of Prussian carp ova in artificial media.
Journal Title: Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society - Year 2010, Vol 3, Issue 4
Abstract
In order to study the effects of storage media and time of storage on the viability of unfertilized eggs of Prussian carp, Carassius gibelio (Bloch, 1782), the ova of this fish, outside the ovary (ex situ storage), were stored in Dettlaff extender (111.3 NaCl + 3.3 KCl + 2.1 CaCl2 + 23.8 NaHCO3) and PCACF (Prussian carp Artificial Coelomic Fluid) medium at 4°C and 21°C for 20, 30, 60 and 120 min. For mimicking of ovarian fluid and designing the PCACF medium, obtained ova from 15 female, were filtered and coelomic fluid was separated for chemical composition analysis of ovarian fluid and the PC ACF medium was formulated. This study was done with 16 treatments and 1 control group in 3 replicate. The results shown that maintain duration and storage medium had significant effect on eyed embryos rate, hatching success (P<0.05), but their effect on malformation of larvae was not significant (P>0.05). Also, temperature had significant effects on percentage of eyed embryos and hatching rate (P<0.05) but no significant effects on number of malformed larvae (P>0.05). Eyed embryo and hatching rates decreased with increasing time. Interactions between time of storage and storage medium and temperature and the resulting effects on eye embryos and hatching rates were significant (P<0.05). Highest eyed embryo and hatching rate between treatments was observed in PCACF, 20 min storage and 4 °C (PCACF + 20 min + 4 °C) and lowest eyed embryo and hatching rates was observed in Dettlaff +120 min + 21 °C.
Authors and Affiliations
Mohammad Taati, Ali Shabani
Keywords
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