SIMULTANEOUS DETERMINATION OF ANAESTHETIC AND ANTIBOTIC IN FLUID BY ELECTOCHEMILUMINESCENCE COUPLING CAPILLARY ELECTROPHORESIS
Journal Title: WORLD JOURNAL PHARMACY AND PHARMACEUTICAL SCIENCE - Year 2018, Vol 7, Issue 4
Abstract
The coupling of electrochemiluminescence (ECL) with capillary electrophoresis (CE) was developed for the simultaneous determination of lidocaine and lomefloxacin in human urine. Under the optimized conditions: ECL detection at 1.15V, 20mM phosphate buffer at pH 6.7, 5mM Ru(bpy)3 2+ and 60mM phosphate buffer at pH 7.6 in the detection reservoir, detection limits of 0.02ug mL-1 for lidocaine and 0.06ug mL-1 for lomefloxacin were obtained. Relative standard derivations of the ECL intensity and the migration time were 3.5 and 1.1% for 6ug mL-1 lidocaine, 3.2% and 3.7% and 1.2% for 6ug mL-1 lomefloxacin, respectively. Developed method was successfully applied to determine the amounts of lidocaine and lomefloxacin in human urine. A baseline separation for lodocaine, proline and lomefloxacin was achieved within 10 mins.
Authors and Affiliations
Su Ming
Keywords
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