SIMULTANEOUS DETERMINATION OF METFORMIN, LINAGLIPTIN IN JENTADUETO AND METFORMIN, SAXAGLIPTIN IN KOMBIGLYZE BY LC-MS METHOD
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 3
Abstract
Objective: The objective of the present investigation was to develop a novel, simple and economic method for the determination of metformin (MET), linagliptin (LIN) and saxagliptin (SAX) in jentadueto and kombiglyze sample by employing the liquid chromatography and mass spectrometric method for estimation in bulk and pharmaceutical dosage form in presence of degradation products.Methods: The chromatographic separation was achieved by using the mobile phase composition of methanol and ammonium acetate buffer pH 4.5 (85:15 % v/v) on the Hypurity advance C-18 column at a flow rate of 0.5 ml/min. Ion signals m/z “130.10/70.10, 473.10/420.40 and 316.30/180.20†for metformin, linagliptin and saxagliptin respectively measured in positive ion mode. The detailed validation of the method was performed as per ICH guidelines.Results: The results of all validation parameters found within acceptance limits. The linearity of the drugs was found to be in the concentration range of 50–5000 ng/ml for all the drugs. Accuracy of the drugs was found to be from 94-102% and precision was found 4.67% RSD for all three drugs. The validated method was employed for the determination of drugs in the formulation and also determined the drugs in the presence of degradation products under stress conditions.Conclusion: The method was developed and validated as per guidelines. Hence, this method can be used for the simultaneous determination of metformin, linagliptin and metformin, saxagliptin in bulk and combined dosage forms.
Authors and Affiliations
P. B. n. Prasad, K. Satyanarayana, G. Krishna Mohan
Keywords
Related Articles
- EP ID EP567556
- DOI 10.22159/ijpps.2018v10i3.23187
- Views 66
- Downloads 0
A RARE CASE OF YELLOW PHOSPHOROUS POISONING WITH ACUTE CHOLESTATIC HEPATITIS, BICYTOPENIA AND IMPENDING HEPATIC FAILURE
Yellow phosphorus is a protoplasmic poison (a potent hepatotoxin). Fulminant poisoning results from ingestion of more than 1 to 2 g. An 18 y old adult male presented with ingestion of rodenticide poison containing 3 perc...
A NOVEL SYNTHESIS OF 4-(5-SUBSTITUTED AMINOMETHYL)-1H-TETRAZOL-1-YL) BENZONITRILES
Objective: Synthesis of novel 4-(5-substituted amino methyl)-1H-tetrazol-1-yl)benzonitriles from p-aminobenzonitrileMethods: A series of novel 4-(5-substituted amino methyl)-1H-tetrazol-1-yl)benzonitriles 6(a-f) were pre...
Stability Indicating Spectrophotometric Methods Determination of Nicardipine in the Presence of its Alkaline Induced Degradation Products
Objective: Derivative, ratio spectra derivative and ratio difference spectrophotometric methods were developed and validated for simultaneous determination of Nicardipine (NIC) in the presence of its alkaline induced deg...
ANTIMICROBIAL EFFICACY OF THE ETHNOMEDICINAL PLANT SPECIES, CANARIUM STRICTUM ROXB
Objective: This study is aimed to evaluate the antimicrobial activity of alcoholic leaf extracts of the ethno medicinal plant species, Canarium strictum belongs to the family, Burseraceae used by Irula tribal community o...
A RETROSPECTIVE STUDY: THE OFF-LABEL USE OF ANTICONVULSANTS AT A PRIVATE HOSPITAL IN INDONESIA
Objective: Anticonvulsant is one class of drugs often used off-label. This study was conducted to investigate the prevalence and the indication of the off-label use of anticonvulsants in a private hospital in Java, Indon...