SIMULTANEOUS DETERMINATION OF METFORMIN, LINAGLIPTIN IN JENTADUETO AND METFORMIN, SAXAGLIPTIN IN KOMBIGLYZE BY LC-MS METHOD
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 3
Abstract
Objective: The objective of the present investigation was to develop a novel, simple and economic method for the determination of metformin (MET), linagliptin (LIN) and saxagliptin (SAX) in jentadueto and kombiglyze sample by employing the liquid chromatography and mass spectrometric method for estimation in bulk and pharmaceutical dosage form in presence of degradation products.Methods: The chromatographic separation was achieved by using the mobile phase composition of methanol and ammonium acetate buffer pH 4.5 (85:15 % v/v) on the Hypurity advance C-18 column at a flow rate of 0.5 ml/min. Ion signals m/z “130.10/70.10, 473.10/420.40 and 316.30/180.20†for metformin, linagliptin and saxagliptin respectively measured in positive ion mode. The detailed validation of the method was performed as per ICH guidelines.Results: The results of all validation parameters found within acceptance limits. The linearity of the drugs was found to be in the concentration range of 50–5000 ng/ml for all the drugs. Accuracy of the drugs was found to be from 94-102% and precision was found 4.67% RSD for all three drugs. The validated method was employed for the determination of drugs in the formulation and also determined the drugs in the presence of degradation products under stress conditions.Conclusion: The method was developed and validated as per guidelines. Hence, this method can be used for the simultaneous determination of metformin, linagliptin and metformin, saxagliptin in bulk and combined dosage forms.
Authors and Affiliations
P. B. n. Prasad, K. Satyanarayana, G. Krishna Mohan
Keywords
Related Articles
- EP ID EP567556
- DOI 10.22159/ijpps.2018v10i3.23187
- Views 46
- Downloads 0
DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF METOPROLOL SUCCINATE AND CILNIDIPINE IN BULK AND PHARMACEUTICAL DOSAGE FORM
Objective: Development and validation of stability indicating RP-HPLC method for the simultaneous estimation of Metoprolol succinate and Cilnidipine in bulk and pharmaceutical dosage form.Methods: The separation was carr...
AMELIORATIVE EFFECT OF CAROB AQUEOUS EXTRACT ON WATER PIPE SMOKE INDUCED-TOXICITY IN ADULT MALE ALBINO RATS
Objective: The aim of the present study was to investigate the daily effect of pre and post treatment of carob aqueous extract (600 mg/kg b. wt.); on rats exposed to were exposed to water pipe smoke (10 mg/kg; 15 minutes...
STUDY ON AVAILABILITY AND AFFORDABILITY OF ANTI HYPERTENSIVE MEDICINES IN THE STATE OF KERALA
Objective: Since the 1970s, one of the smallest Indian States, Kerala, has been internationally applauded for its excellent performance in its health indicators. Even if mortality is low, the morbidity (those suffering f...
METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF SERTRALINE AND DOXOFYLLINE IN PHARMACEUTICAL DOSAGE FORM BY RP-HPLC
Objective: The aim of this work was to develop and validate a simple Reversed Phase - High Performance Liquid Chromatography method for the simultaneous estimation of Sertraline and Doxofylline in pharmaceutical dosage f...
FABRICATION OF NANOFIBRES BY ELECTROSPINNING USING KERATIN FROM WASTE CHICKEN FEATHERS, PVA AND AgNPs
Objective: To prepare and characterise keratin from chicken feathers (CF), collected from the slaughter house, and to blend with poly vinly alcohol (PVA) and biosynthesised silver nanoparticles (AgNPs) and to convert int...