SIMULTANEOUS ESTIMATION OF IRBESARTAN AND HYDROCHLOROTHIAZIDE BY UV SPECTROSCOPY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 6
Abstract
Objective: The literature survey revealed that various methods are reported for determination of Irbesartan and Hydrochlorothiazide alone or in combination with other drugs So, the main objective of our study was to develop simple, accurate and reproducible methods for the simultaneous estimation of Irbesartan and Hydrochlorothiazide in a combined dosage form.Methods: The method for the simultaneous determination of Irbesartan and Hydrochlorothiazide by spectroscopy has been developed. The simple, accurate and precise method includes Area Under the Curve (AUC) method. On response to the effect of solvent on spectral behaviors of Irbesartan and Hydrochlorothiazide, methanol was selected as solvent. Irbesartan shows maximum absorbance at 224 nm and Hydrochlorothiazide shows maximum absorbance at 271 nm. For the AUC method, the wavelength ranges between 225-230 nm and 258-265 nm respectively were selected with reference to the absorbance curves plotted between the wavelengths of 200-400 nm. This method allows rapid analysis of two drug combinations.Results: The result of analysis was validated statistically by recovery study following ICH method validation guideline. Tablet containing both drugs was assayed using the method developed, showing a good accuracy and precision.Conclusion: It can therefore be concluded that use of this method can save more time and money and it can be used in small laboratories with accuracy
Authors and Affiliations
Piusha Shakya, Pushpendra Kumar Jain, S. P. Shrivastava, Asmita Gajbhiye
Keywords
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