Simultaneous Estimation of Luteolin and Apigenin in Methanol Leaf Extract of Bacopa monnieri Linn by HPLC
Journal Title: Journal of Pharmaceutical Research International - Year 2014, Vol 4, Issue 13
Abstract
Aims: To develop, validate and quantify the content of flavonoids luteolin and apigenin in aerial parts of methanol leaf extract of Bacopa monnieri (B. monnieri) by reverse phase liquid chromatography. Study Design: High Performance Liquid Chromatography Place and Duration of Study: Department of Pharmaceutical Analysis, KMCH College of Pharmacy, Coimbatore and Indian Pharmacopoeia Commission, Ghaziabad, India from 1.7.2010 to 30.6.2011. Methodology: Separation and quantification of flavonoids was performed on a C18 column (5µm, 200mm×4.6mm, id.) using potassium dihydrogen phosphate buffer (20mM, pH 3.5 adjusted with ortho phosphoric acid, v/v) and acetonitrile as mobile phase with a flow rate of 1ml/min. The column effluents were monitored at 348nm with column temperature kept at 30±1ºC. Results: A validated method for simultaneous estimation of luteolin and apigenin was developed, where limits of detection and quantification was found to be 0.03 and 0.91µg/ml for luteolin, 0.041 and 0.13µg/ml for apigenin respectively. The percentage of these phytoconstituents recovered was in the range of 98.07-99.71 (%RSD<2%). Conclusion: The developed validated HPLC method was found to be accurate, precise and robust and may be used for analysis of luteolin and apigenin in the extracts of B. monnieri.
Authors and Affiliations
Aiyalu Rajasekaran, Nagulsamy Sarathikumar, Vivekanandan Kalaiselvan, Muthusamy Kalaivani
Keywords
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- EP ID EP344859
- DOI 10.9734/BJPR/2014/9009
- Views 108
- Downloads 0
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