Simultaneous Estimation of Luteolin and Apigenin in Methanol Leaf Extract of Bacopa monnieri Linn by HPLC
Journal Title: Journal of Pharmaceutical Research International - Year 2014, Vol 4, Issue 13
Abstract
Aims: To develop, validate and quantify the content of flavonoids luteolin and apigenin in aerial parts of methanol leaf extract of Bacopa monnieri (B. monnieri) by reverse phase liquid chromatography. Study Design: High Performance Liquid Chromatography Place and Duration of Study: Department of Pharmaceutical Analysis, KMCH College of Pharmacy, Coimbatore and Indian Pharmacopoeia Commission, Ghaziabad, India from 1.7.2010 to 30.6.2011. Methodology: Separation and quantification of flavonoids was performed on a C18 column (5µm, 200mm×4.6mm, id.) using potassium dihydrogen phosphate buffer (20mM, pH 3.5 adjusted with ortho phosphoric acid, v/v) and acetonitrile as mobile phase with a flow rate of 1ml/min. The column effluents were monitored at 348nm with column temperature kept at 30±1ºC. Results: A validated method for simultaneous estimation of luteolin and apigenin was developed, where limits of detection and quantification was found to be 0.03 and 0.91µg/ml for luteolin, 0.041 and 0.13µg/ml for apigenin respectively. The percentage of these phytoconstituents recovered was in the range of 98.07-99.71 (%RSD<2%). Conclusion: The developed validated HPLC method was found to be accurate, precise and robust and may be used for analysis of luteolin and apigenin in the extracts of B. monnieri.
Authors and Affiliations
Aiyalu Rajasekaran, Nagulsamy Sarathikumar, Vivekanandan Kalaiselvan, Muthusamy Kalaivani
Keywords
Related Articles
- EP ID EP344859
- DOI 10.9734/BJPR/2014/9009
- Views 102
- Downloads 0
Effects of Wild Artemisia herba-alba Essential Oil on Biofilm-Forming Bacteria
Aims: The aim of the present study was to determine the effects of essential oil of wild Artemisia herba-alba grown in south Jordan on biofilm-forming bacteria. Study Design: Seven bacterial clinical isolates were used i...
Synthesis of Selenium Nanoparticles by Bacillus laterosporus Using Gamma Radiation
Aim: This study shows the possible synthesis of Selenium Nanoparticles (SeNPs) in aerobic optimized conditions using Bacillus laterosporus (B. laterosporus) bacterial strain. Methodology: B. laterosporus was used to redu...
In vitro Anticancer Activity of Hibiscus vitifolius Flowers Ethyl Acetate Fraction against Hepg2 Cell Line
Objective: The roots of Hibiscus vitifolius (Malavaceae) are used for the treatment of jaundice in the folklore system of medicine in India. This study is an attempt to evaluate anticancer activity of the flowers of Hibi...
Post Autologous Stem Cell Transplantation Maintenance for Multiple Myeloma Patients: Real World Experience and Results
Maintenance therapy is routinely prescribed for multiple myeloma (MM) patients after autologous stem cell transplantation (ASCT). In this retrospective analysis, we evaluated 257 post-ASCT MM patients and compared the ef...
Evaluation of an Undocumented Polyherbal (Faradin®) Used for the Treatment of Sickle Cell Disease in West Africa. Part II: Antibacterial Activity and Synergism
Aim: This study focused on evaluation of the anti-bacterial effects and synergism of Faradin®), a polyherbal complementary alternative medicine (CAM) and anti-sickling agent. It is used for treatment of sickle cell disea...