Solvent Optimization For Genistein Isolation Of “Rotten Tempe” By High Performance Liquid Chromatography Method
Journal Title: Eksakta: Jurnal Ilmu-Ilmu MIPA - Year 2017, Vol 17, Issue 2
Abstract
Genistein is a soy isoflavone that has been known have anticancer properties. The aim of this research is to determine the right combination of solvents to extract isoflavones in rotten tempe and determine the genistein content of the extractionprocess by the High Performance Liquid Chromatography (HPLC) method. Isoflavone extraction process was done using a mixture of methanol and chloroform solutions. Isoflavone extracts obtained will be analysed usingHigh Performance Liquid Chromatography (HPLC) method to determine the content of genistein.The optimum isoflavone extract was obtained from chloroform:methanol (10:1 v/v)solvent ratio. Where as the highestcontentsof genistein from the 4thday “rotten tempe” as 26.199± 25.146 (mg/g).
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- EP ID EP545449
- DOI 10.20885/eksakta.vol17.iss2.art3
- Views 139
- Downloads 0
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