Somatic embryogenesis in Musa AAAB, cv. FHIA-18 using liquid culture media
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 2
Abstract
Homogenous cell suspensions were iniciated from somatic embryos in the globular stage and the greatest volume of cell biomass on multiplying the suspensions at a density of 3.0% PCV. From the fifteenth day in culture medium for the formation of embryos, structures consisting of proembryos and somatic embryos in the globular stage started to form. With respect to the densities studied, the best results were obtained with 100 mgFW, where 1 871 SE.l-1 formed with a weight of 248 mgFW.l-1 after 30 days. With an initial density of 0.6 gFW in the culture medium for secondary multiplication, an increase of 42.9-fold the initial amount of fresh weight was obtained; after 60 days of culture 15 985 SE.l-1 were obtained. The greatest percentage of maturation was obtained with 400 mgFW with 70% of mature somatic embryos. The positive effect of Biobras-6 (brassinosteroid analogous) was confirmed, with a concentration of 0.01 mg.l-1 the best germination percentages were obtained in liquid and semisolid culture medium. Embryo germination in temporaly inmersion (RITA) was achieved with an inoculum density of 0.5 gFW for system with 85% germination. One thousand plants obtained from somatic embryos were taken to ex vitro environment, along with plants derived from conventional micropropagation (shoot tips) to carry out studies on the possible presence of somaclonal variation. During the first cycle of production, the plants derived from the two methods in vitro culture showed differences with respect to the plants derived from corms in height, diameter and number of suckers. In the second production cycle, the plants from somatic embryos showed similar characteristics to the plants derived from shoot tip and corms with respect to the morphological parameters evaluated, with only 0.2% of the plants with phenotypic changes.
Authors and Affiliations
Luis A. Barranco, Rafael Gómez-Kosky, Maritza Reyes, Boris Chong
Keywords
Related Articles
- EP ID EP697795
- DOI -
- Views 68
- Downloads 0
Tissue culture and genetic transformation in Glycine max (L.) Merrill
This work is a brief overview on the background of in vitro tissue culture and genetic transformation of soybean, as well as a brief introduction to the origin, distribution and importance of the crop. It is also aimed...
Use of Temporary Immersion Systems (RITA) in the propagation of plants via organogenesis in sugar cane and bananas
The present work had as objective to study the possibilities to use the RITA, in the micropropagation plant via axillary buds of sugarcane and of the banana hybrid (Musa sp. AAAA cv. FHIA-01 and AAAB cv. FHIA-18). Two d...
Production of taxoids in callus and cell suspensions of Taxus chinensis through the use of abiotic elicitors
Until now, several strategies have been developed to improve the production of secondary metabolites using plant cell cultures, manipulating the parameters of the environment, selecting high yielding cell clones and the...
Exploring biocontrol efficacy of Trichoderma spp. against Fusarium sacchari, the causal agent of sugarcane wilt
Wilt caused by Fusarium sacchari, is one of the major diseases of sugarcane (Saccharum spp.) causing considerable economic loss. Trichoderma spp. are important biocontrol agents which have been exploited effectively for...
Rhizosphere of three cultivars of Phaseolus vulgaris L. source of endospore-forming bacteria with biotechnological potential
Plant Growth Promoting Rhizobacteria constitute a promising source of microorganisms, with the capacity to develop new biopesticides that contribute to pest control, with an agroecological and sustainable basis. The obje...