Structure of the chorioretinal complex in the rabbit eye after vitrectomy. Report 2. Vitreous cavity irrigation with different temperature solutions for 60 minutes
Journal Title: Офтальмологический журнал - Year 2018, Vol 0, Issue 4
Abstract
Background: It remains poorly understood what should be the temperature of the irrigation solution for intraocular surgery and how long it is reasonable to use irrigation solutions during vitrectomy. Purpose: To investigate the structure of rabbit chorioretinal complex after vitrectomy with the use of irrigation solutions having different temperatures for 60 minutes. Materials and Methods: Twelve Chinchilla rabbits (24 eyes) were divided into two experimental groups and one control group. The two experimental groups, each of 5 rabbits (10 eyes), underwent a three-port pars plana vitrectomy with either 22°C or 5°C irrigation solution. Two intact rabbits (4 eyes) were used as controls for comparison. The duration of irrigation/aspiration was 60 minutes. Specimens for histology were obtained at days 1 and 7 after surgery. The chorioretinal complex (i.e., the choroid and retina) was histologically examined. Results: After vitrectomy with 60-minute continuous vitreous cavity cooling with either the 22°C or 5°C irrigation solution, we found retinal structural changes in the form of uneven edema, (a) predominantly in the inner retinal layers, at day 1, and (b) in both the inner and outer retinal layers, at day 7. There were destructive changes in the inner retinal layers on some slides from rabbits of both groups. In addition, the choroid appeared somewhat thickened. Conclusion: In the rabbit eyes after vitrectomy with 60-minute continuous vitreous cavity cooling with either the 22°C or 5°C irrigation solution, we found the uneven retinal edema that is likely to be mediated by aseptic inflammatory response to cooling-induced and mechanical damage to the retina, and might be also associated with the composition of the irrigation fluid.
Authors and Affiliations
O. Zadorozhnyy, R. Nazaretian, V. Myrnenko, V. A. Naumenko, E. Maltsev, N. V. Pasyechnikova
Keywords
Related Articles
- EP ID EP423707
- DOI 10.31288/oftalmolzh201844955
- Views 84
- Downloads 0
Changes in the retinal thickness after microinvasive vitrectomy with inner limiting membrane removal in patients with diabetic retinopathy
The purpose. To study changes in the macular retinal thickness in patients with diabetic retinopathy after microinvasive vitrectomy with inner limiting membrane (ILM) removal. Material and Methods. This was a retrospect...
Criteria of severity for macular edema associated with retinal vein occlusion: cohort heterogeneity and efficacy evaluation of threshold and subthreshold retinal laser photocoagulation depending on the macular edema severity criteria. Part II.
Background. Macular edema (ME) develops in 60-100% of patients with retinal vein occlusion (RVO). ME becomes chronic with a decrease in visual acuity in 1/3-2/3 of patients with RVO-associated ME. The gold standard of ca...
Structure of the chorioretinal complex in the rabbit eye after vitrectomy. Report 1. Vitreous cavity irrigation with different temperature solutions for 30 minutes
Background: It remains poorly understood what should be the temperature of the irrigating solution for intraocular surgery and how long it is reasonable to use irrigating solutions during vitrectomy. Purpose: To invest...
Changes in immunity characteristics in patients with small (T1) choroidal melanoma after a course of 810-nm diode laser TTT delivered using the developed methodology
Background: Immune response is an important factor for efficacy of choroidal melanoma (CM) treatment. Adequate uveal melanoma (UM) organ-saving treatment and treatment efficacy monitoring requires determining the interac...
Oxidation and peroxidation in the uvea of the rabbit eyes with experimental uveitis and ocular hypertension
Background. The role of metabolic changes in the anterior eye tissues in the pathogenesis of uveal inflammation in patients with elevated intraocular pressure has been studied poorly. Of particular interest, in this resp...