STUDIES ON HEAVY METAL TOLERANCE AND ANTIBIOTIC RESISTANCE PATTERNS OF BACTERIAL POPULATION ISOLATED FROM EFFLUENT TREATED WATER OF DELHI
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2013, Vol 2, Issue 5
Abstract
Along with the growing industrialization, problem of toxic heavy metal contamination is increasing worldwide. Microbes related technologies may provide an alternative or addition to conventional method of metal removal or metal recovery. The present study dealt with isolation, identification and characterization of microorganisms showing tolerance towards several heavy metals and resistance against antibiotics. These bacterial strains were isolated from different effluent treatment plants located in suburb of Delhi. Initially samples were analyzed for the presence of B.cereus, P.aeruginosa, E. coli & S.aureus through biochemical and morphological methods. Primary screening methods were applied to isolate heavy metals (viz. lead, zinc and cadmium) resistant organisms. Further susceptibility patterns of these isolates were studied towards clinically significant antibiotics such as meropenem, Doxycycline, streptomycin, tetracycline, gentamycin, cloxacillin, amoxicillin, ampicillin, vancomycin, and cephalosporin. All isolates were found to have 100% susceptibility against cadmium & 100% resistance against lead except P.aeruginosa (67%). Doxycycline was found to be most inert antibiotic as it was ineffective against all isolates, whereas meropenem was found to be most promising antibiotic followed by cephalosporin, streptomycin, tetracycline, and gentamycin. These heavy metal resistant organisms could be a potential agent for bioremediation of heavy metals polluted environment.
Authors and Affiliations
Shraddha Sinha*| Department of Microbiology, Shriram Institute for Industrial Research 19, University road, Delhi.-11007, India, Rudrangshu Chatterjee| Department of Microbiology, Shriram Institute for Industrial Research 19, University road, Delhi.-11007, India, Dushyant Singh| Department of Microbiology, Shriram Institute for Industrial Research 19, University road, Delhi.-11007, India, Amita Gaurav Dimri| Department of Microbiology, Shriram Institute for Industrial Research 19, University road, Delhi.-11007, India, ML Aggarwal| Department of Microbiology, Shriram Institute for Industrial Research 19, University road, Delhi.-11007, India
Keywords
Related Articles
- EP ID EP2294
- DOI -
- Views 354
- Downloads 30
Drug Utilization Pattern of Anesthetics in a Tertiary care Hospital
Background: Anesthesia means ‘loss of sensation’. Medications that cause anesthesia are called anesthetics. Anesthetics are used during tests and surgical operations to induce sleep, which prevents pain and discomfort an...
“A Large Incidentally-Diagnosed Left-Lobed Hepatic Hydatid Cyst in a Young Male Patient”
Introduction: Despite modern healthcare facilities, hydatid disease (HD) remains a perpetuating public health problem in endemic countries. Infection with Echinococcus may have a diverse clinical evolution, leading even...
UNDISPUTABLE BEHAVIOUR OF LIPID PROFILE IN CHOLELITHIATIC GALL BLADDER
In recent years, effort has been made to knowing the pathophysiological basis of gallstone formation. The role of serum lipids in the etiology of cholelithiasis is very important and in cholesterol gallstones serum lipid...
Immunoprecipitation of 11 ZN fingers Domains, CTCF and BORIS to Multifunctional Y-box DNA/RNA-binding factor, YB-1 in Glioma-RGBM
CCCTC binding factor (CTCF) is a unique highly conserved and ubiquitously expressed 11 zinc finger (ZF) transcriptional factor with multiple target site. It is able to bind to various target sequences to perform differen...
Strategic Approaches of Controlling Variables in RP-HPLC Method Development and Validation: A Review
High Performance Liquid chromatography is the principal technique in accessing the drug, its various impurities and drug related degradants that can form on synthesis or storage in pharmaceutical products. It results in...