Studies on Molecular Identification of Anabaena Cyanobionts of Azolla Species
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 6
Abstract
Azolla is a genus of free-floating water fern which is widely distributed in aquatic habitats of the tropics and temperate regions. It is one of few vascular plants known to exhibit a symbiotic association with N2- fixing blue - green algae, Anabaena sp. In view of this, a study was carried out on molecular identification of Anabaena strains isolated from three Azolla species. The selected Azolla species were grown in culture medium prepared with garden soil, goat manure, super phosphate and its combinations. The three strains of Anabaena cyanobiants present in the leaf cavity of three different Azolla species were isolated and grown in BG11 medium. Out of three Anabaena isolates, only two isolates (i.e., strains -1 & 2 isolated from Azolla microphylla and A.filiculoides respectively), were able to grow an BG11 medium while other strain isolated from A.pinnata have not grown on the culture medium. These two Anabaena cyanobiants were further mass cultured in BG11 medium and were identified through molecular studies using 23S r RNA sequencing. Thus, the two selected strains were identified as Anabaena sphaerica (KX055938) and A.variabilis (KX055939).
Authors and Affiliations
Muniappan. K, Mahalingam. P. U, Therasammal. M
Keywords
Related Articles
- EP ID EP387113
- DOI 10.9790/3008-1106055356
- Views 60
- Downloads 0
Anti-Cancer Therapy: Chlorogenic Acid, Gallic Acid and Ellagic Acid in Synergism
Phytochemicals have been used as effective agents for treating illnesses. Its use has been widely increased in the present scenario where commercial drugs are carrying a huge burden of side effects. Many phytochemicals h...
A Study On Effect Of Pharmacist Mediated Patient Counseling In Hypertensive Patients In Terms Of Knowledge, Compliance And Quality Of Life.
To create awareness about hypertension in order to improve their quality of life. Free health camps were conducted; screening of blood pressure was done. Data were collected based on medical history, family history, food...
Anti-Diabetic Activity Of Polyherbal Formulation On Alloxan Induced Diabetes.
Diabetes is a metabolic disorder with major complication associated with hyperglycemia, inflammation, foot ulcer, Nerve disorders and sexual depression Plant medicines are readily used in combination rather than in a sin...
Study of Malaria Vectors Density Using Artificial Breeding Container in Yola-South L.G.A of Adamawa State
Mosquitoes are vectors for many pathogens with catastropic effects on global heath. A total of 1806 mosquitoes were sampled from earthen pots and examined under a microscope. Distribution of malaria vectors density by si...
Biological investigations of different leaf extracts of Litsea liyuyingi (Family-Lauraceae)
In this present study, the leaf extracts of Litsea liyuyingi, were subjected to evaluate the phytochemical screenings, thrombolytic, membrane stabilizing, antioxidant, antimicrobial, cytotoxic, antinociceptive, anti-diar...