Study of culture media for the in vitro conservation of cassava
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 4
Abstract
Plant tissue culture constitutes an alternative for germplasm conservation in case of vegetatively propagated species. This approach permits to maintain collections in small places free from attach of diseases and catastrophes. Eleven variants from MS culture media were tested. Variants consist of different sucrose (20, 30 and 40 g.l-1) and manitol (0, 10, 20 y 30 g.l-1) concentrations in order to decrease the subculture number in the in vitro storage of Señorita and CEMSA 74-725 clones. Evaluations were carried out nine months after in vitro implantation based on: height (cm), internode number by plant, number of active leave, number of active roots and surviving percentage. After storage, explants were incubated for recovery in the described culture medium for in vitro cassava growing. A culture medium with the addition of 40 g.l-1 sucrose, 0.02 mg.l-1 BAP, 0.1 mg.l-1 de GA3 and 0.01 mg.l-1 ANA is recommended.
Authors and Affiliations
Aymé Rayas, Victor Mederos, Magaly García, Jorge López, Manuel Cabrera, José de la C. Ventura, Marilín Martínez, Valentina Gutiérrez, M. Álvarez, Maricel Bauta
Keywords
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