Obtaining somatic embryos and establishing embryogenic cell suspensions in the banana cultivar 'Navolean' (AAB)
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 2
Abstract
Cells suspension of plantains and bananas with promising results have been reported internationally, however, in Cuba it is not at so for AAB group. So, the following working objectives have to be considered: in vitro multiplication of the material used as explant source. For in vitro multiplication of the material used as explant source. For in vitro multiplication of the material, several 6-BAP and IAA concentration were studied. Induction of embryogenic cultures was the developed form scalps incubated in solid medium ZZ. Suspensions were established in 10 ml and 25 ml Erlenmeyers containing liquid medium ZZ. The best medium for explant multiplication was MS (salts and vitamins), additional thiamin (1 mg.l-1), sucrose (40 g.l-1); 4.50 mg.l-1 6-BAP, 0.88mg.l-1 IAA and solidified agar (6.0 g.l-1) (medium 7). A 4.66% callus formation with embryogenic cultures was obtained, and cell suspensions were established 20 days after incubation in 10 ml Erlenmeyers. Media were changed every third day.
Authors and Affiliations
Arletys Santos, Jorge López, Manuel Cabrera, Nery Montano, Damisela Reinaldo, José de la C. Ventura, Victor Medero, Magalys García, Milagros Basail, Ayme Rayas
Keywords
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