In situ genomic DNA extraction for PCR analysis of regions of interest in four plant species and one filamentous fungi
Journal Title: Biotecnología Vegetal - Year 2014, Vol 14, Issue 3
Abstract
The extraction methods of genomic DNA are usually laborious and hazardous to human health and the environment by the use of organic solvents (chloroform and phenol). In this work a protocol for in situ extraction of genomic DNA by alkaline lysis is validated. It was used in order to amplify regions of DNA in four species of plants and fungi by polymerase chain reaction (PCR). From plant material of Saccharum officinarum L., Carica papaya L. and Digitalis purpurea L. it was possible to extend different regions of the genome through PCR. Furthermore, it was possible to amplify a fragment of avr-4 gene DNA purified from lyophilized mycelium of Mycosphaerella fijiensis. Additionally, it was possible to amplify the region ap24 transgene inserted into the genome of banana cv. ‘Grande naine’ (Musa AAA).
Authors and Affiliations
Luis E. Rojas , Maritza Reyes , Naivy Pérez-Alonso , María I. Olóriz , Laisyn Posada-Pérez, Bárbara Ocaña , Orelvis Portal , Borys Chong-Pérez , Jorge L. Pérez Pérez
Keywords
Related Articles
- EP ID EP683570
- DOI -
- Views 129
- Downloads 0
An alternative to estimate the retention of atmospheric carbon by Bambusa vulgaris var. Schrad vulgaris. ex J.C. Wendl.
The estimation of atmospheric carbon sequestration by a woody plant has two alternatives for implementation: by identifying the average annual increase in volume or by determining the appropriate method for calculating...
In vitro propagation of Hylocereus purpusii Britton & Rose, a mexican species in danger of extinction
The purpose of this work was to evaluate different conditions and culture parameters for the in vitro establishment and multiplication of Hylocereus purpusii. Seeds were used as plant material and a workflow was develope...
Field response of Eucalyptus grandis W. Hill ex Maiden plants obtained by micropropagation and seeds
The use of plants obtained by in vitro culture could contribute to satisfy the demand of products derived from this plant in the agricultural sector. However, the response of plants in the field is not well known. The...
Effect of heat treatment at high temperature on in vitro seeds germination of Phaseolus vulgaris cv. ‘ICA Pijao’
The common bean (Phaseolus vulgaris L.) production is limited by biotic and abiotic factors. Among them, water stress, saline or high temperatures. To solve this problem, genetic improvement programs are aimed at the s...
In vitro establishment of Eugenia squarrosa: an endemic species of Santa Clara (Cuba) in n extinction danger
The tissue culture techniques can be supplemented with others that are carried out in the cultivation in situ and being applied in combined way to give solution to the extinction of different species. Eugenia squarrosa...