Study on the growth inhibitory effects of Puromycin and Doxorubicin on tumor cell lines (A549, HepG2, HT29 and K562) using Cell Titer-Glo and Alamar blue (Resazurin) based cell viability assays
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2014, Vol 2, Issue 1
Abstract
Cancer is a life-threatening spectrum of diseases in which mortality rates are high. There is more money being spent worldwide on In-vitro cancer research. Cell viability assay is the initial step to develop the anticancer drug. Here, we have used four different cancer cell lines (A549, HepG2, HT29 and K562) to understand the viability behaviour of different cancer against Puromycin and Doxorubicin. Before starting the vitality assay, cells were revived and maintained. Growth curve experiment were followed in 48 wells plate and counted for 4 days with every 24Hrs intervals. Haemocytometer was used for cell counting and based on the cell count data graph were plotted using prism software. CellTiter Glo viability assay and Resazurin viability assay were done to all the four cell lines. Envision luminescence plate reader used since both are luminescence based assays. Luminescence reading was converted to graphical data by using Prism software. Regarding the Growth curve doubling time is more to A549 and HT29 when compared to HepG2 and HT29. According to viability assays, IC50 of Puromycin and Doxorubicin were determined.
Authors and Affiliations
Praveen Kumar Ravichandran, Manoj. P
Keywords
Related Articles
- EP ID EP33190
- DOI -
- Views 266
- Downloads 0
Formulation, characterization and evaluation of zidovudine controlled release matrix tablets using HPMC K4M and K100M
The aim of present investigation is preparation, characterization and evaluation of oral controlled release matrix tablets of Zidovudine (AZT) in order to improve efficacy and reduce its side effects. FTIR and DSC conf...
Analytical method development and validation for the simultaneous estimation of Montelukast and Doxofylline in bulk and pharmaceutical dosage forms by using RP-HPLC
The present investigation describes about a simple, economic, selective, accurate, precise reverse phase high performance liquid chromatographic method for the simultaneous estimation of Montelukast and Doxofylline in...
Development and validation of a stability indicating HPLC method for analysis of Altretamine in bulk drug and pharmaceutical formulations
A simple, sensitive and accurate stability indicating HPLC method has been developed and validated for determination of Altretamine in its bulk form and pharmaceutical formulations. Chromatographic separation was achie...
A prospective observational study to measure drug compliance in epilepsy patients attending Chakrapani neuro care hospital
Purpose: Epilepsy may promote limitations and restrain activities, interfering with the occupational ability, professional goals and social integration of patients. It increases morbidity and symptomatic epilepsy reduc...
Study on the effect of different parts of Nyctanthes arbortristis linn. on the hematological parameters of mice
Hematological effect of the ethanolic extracts of the flowers, barks, seeds and leaves of Nyctanthes arbortristis Linn were estimated at the dose levels of 200, 400 and 600 mg/kg body weight (I.P.), which were selected...