Accurate, simple and quantitative UV- Spectroscopic method for the estimation of Sofosbuvir in pure and marketed formulations
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2017, Vol 5, Issue 5
Abstract
A simple, accurate and precise UV spectroscopy method has been developed for the estimation of Sofosbuvir. It is a medication used for the treatment of Hepatitis C. It inhibits the hepatitis CNS 5B protein. It appears to have a high barrier to the development of resistance. Here we have developed a UV spectroscopic method for the quantitative determination of Sofosbuvir in the bulk and tablet dosage form. The parameters i.e., linearity, precision, accuracy, limit of detection and limit of quantification were studied according to international conference on harmonization guidelines (ICH). The determination was carried out at an absorption maximum of 259nm using 0.1N HCl, as solvent. In the present method, linearity over the concentration range of Sofosbuvir was found to be 3-15µg/ml, with a correlation coefficient of 0.9998. The results of analysis have been validated statistically for linearity, accuracy and precision, LOD and LOQ of the processed method. The developed method was successfully applied for the quantitative analysis of commercially available dosage form.
Authors and Affiliations
Kumar Raja Jayavarapu, G. Isaac1, R. Sri Dharani1, S. Prabha, Sk. Raziya, T. Satyanarayana and S. Parimalakrishnan
Keywords
Related Articles
- EP ID EP33488
- DOI -
- Views 382
- Downloads 0
A NOVEL RP- HPLC METHOD DEVELOPMENT AND VALIDATION OF ATORVASTATIN AND FENOFIBRATE IN BULK AND PHARMACEUTICAL DOSAGE FORMS
A fast, robust and accurate RP-HPLC method was developed and validated for simultaneous determination of Losartan potassium and Amlodipine in tablets. The mobile phase was mixture of aqueous Tri ethyl amine with pH 2.0...
Investigation of Oxygen absorbent in food packages
Active packaging is one of the newest concepts in food packages uses in order to supply the customer demands in shortest time. To prepare targeted foods, globalization of food market, transferring to longer distances,...
Nanoparticles for effluent and drinking water treatment
Effluent management has become a crucial challenge due to increased population and industrial pollution in the recent times. Supply of purified drinking water has become a top priority for every country on the globe. T...
FORMULATION, CHARACTERIZATION AND IN-VITRO DISSOLUTION STUDIES OF DICLOFENAC SODIUM TABLETS THROUGH SOLID DISPERSION USING POLY ETHYLENE GLYCOL
In the present study the formulation of Diclofenac sodium tablet from poly ethylene glycol solid dispersion has been carried to enhance the bioavailability and solubility of the drug, Diclofenac. The post compression s...
A validated RP HPLC method for simultaneous estimation of lamivudine and tenofovir disoproxil fumarate
A precise, simple, selective and accurate RP-HPLC method was developed and validated for rapid assay of Lamivudine and Tenofovir fumarate in pharmaceutical dosage form. Lamivudine and Tenofovir disoproxil fumarate are...