Study on the stability of SARS-CoV-2 at different temperatures
Journal Title: Shanghai Journal of Preventive Medicine - Year 2021, Vol 33, Issue 9
Abstract
<b>Objective:To observe the stability of severe acute respiratory syrdrome coronavirus （SARS-CoV-2） in cell cultures at different temperatures so as to provide basic data and scientific basis for the research and control of COVID-19 epidemic. <b>Methods:The Vero E6 cells inoculated with SARS-CoV-2. According to TCID<sub>50</sub>， SARS-CoV-2 with different dilution （10<sup>-1</sup>， 10<sup>-3</sup>， 10<sup>-5</sup>， 10<sup>-6</sup>）were stored at 37 °C， 22.5 °C， and 4 °C for one to seven days， and then infectious titer was determined by micro cytopathogenic effect assay， observing cytopathic effect （CPE）， and real-time fluorescence quantitative testing. <b>Results:SARS-CoV-2 was stable under 4 °C. The infectivity of high concentration （10<sup>-1</sup> dilution） under 22.5 °C for seven days gradually decreased， while lower concentration completely lost infectivity after one day. The virus lost infectivity when stored at 37 °C for more than one day. <b>Conclusion:SARS-CoV-2 is highly stable at 4 °C， sensitive to heat， and related to virus concentration.
Authors and Affiliations
Xiao-xian CUI, Jia-bin MOU, Zheng TENG, Yan-qiu ZHOU, Fang-hao FANG, Hong-you CHEN, Hui JIANG, Chong-shan LI, Min CHEN, Xi ZHANG
Keywords
Related Articles
- EP ID EP709363
- DOI -
- Views 11
- Downloads 0
Burnout prevalence and influencing factors among nurses in a tertiary general hospital in Shanghai
ObjectiveTo investigate the prevalence and influencing factors of job burnout among nurses in a tertiary general hospitalï¼ providing a reference for medical institutions to develop effective management for job burnout....
Prevalence and influencing factors analysis of the polycystic ovary syndrome among female college students in Fuzhou City, Jiangxi Province
Objective To investigate the prevalence of polycystic ovary syndrome （PCOS） among female college students at a university in Fuzhou City， Jiangxi Province， and to facilitate early detection and intervention of PCOS.M...
HIV genotypes and molecular transmission networks among MSM with newly reported HIV infections in Dehong Prefectureï¼ Yunnan Province from 2010 to 2019
ObjectiveThis study aimed to investigate the HIV genotypic subtypes and molecular transmission clusters among men who have sex with men ï¼MSMï¼ with newly reported HIV infections in Dehong Dai and Jingpo Autonomous Pre...
Relative bioavailability of cadmium in aquatic products and its application on the cadmium exposure risk assessment
Objective To understand the relative bioavailability （RBA） of cadmium in different aquatic products. Based on the consideration of the gender differences and the relative bioavailability of cadmium in different foods，...
Social support and parent-child relationship among adolescents and their influence on emotions
ObjectiveTo explore the association between social supportï¼ parent-child relationshipsï¼ and depressionï¼ anxietyï¼ and loneliness among adolescentsï¼ in order to provide a reference for improving mental health in...