Study to identify the role of high performance liquid chromatography in detecting haemoglobinopathies in antenatal patients
Journal Title: Indian Journal of Pathology and Oncology - Year 2018, Vol 5, Issue 1
Abstract
Introduction Haemoglobinopathy is a major genetic public health problems in India responsible for significant morbidity and mortality Individuals with trait carriers are healthy and unaware of their carrier status unless specifically screened If a couple carry a clinically significant haemoglobinopathy trait there is a 1 in 4 chance with each pregnancy that their child will inherit a major haemoglobinopathy The most effective approach to reduce the burden of the society is to reduce the incidence by implementation of a carrier screening programmeMaterial and Methods The present study was carried out in a tertiary care hospital for a period of 2 years 500 Antenatal patients attending Obstetricout patient department for routine checkup were included in the study Screening of patient was done by Solubility test Sickling test and Alkali denaturation test Finally a very standardized method to detect HbA2 and HbF was done by using an automated High Performance Liquid ChromatographyResults Out of a total of 500 pregnant women 4 were having haemoglobinopathies whereas 96 had normal haemoglobin Out of 20 cases maximum number of cases ie 22 was of Thalassemia trait followed by Sickle cell trait cases ie 12 Other haemoglobinopathies are also found ie sickle cell disease 02 Thalassemia case 12 and double heterozygous 02Out of a total of 20 positive pregnant women only partners of 8 women were screened and out of them 375 were found positiveConclusion Detection of carrier status using HPLC during pregnancy along with couple screening provides prospective parents with the option of testing the fetus for hemoglobinopathyKeywords Antenatal Double heterozygous Haemoglobinopathies High Performance Liquid Chromatography Thalassemia trait
Authors and Affiliations
Komal D. Sawaimul, M. Banyameen Iqbal, Vijayalaxmi D. Sawaimul, Tushar Kambale, Rajharsh Hanmante
Keywords
Related Articles
- EP ID EP473172
- DOI 10.18231/2394-6792.2018.0002
- Views 71
- Downloads 0
Age specific prevalence of endometrial hyperplasia in a tertiary care centre from south India
Introduction: Endometrial hyperplasia is a premalignant lesion for endometrial carcinoma accounting for 6% of new female cases and 3% of female cancer deaths.(1,2) Endometrial cancer is the third most common malignancy o...
Diagnostic significance of various cytomorphological features and their quantification for the diagnosis of papillary carcinoma thyroid on fine needle aspiration cytology
Introduction The relative reliability of the morphological features for making a diagnosis of PTC when considered independently is debatable Quantification of morphological features has been attempted to improve the diag...
Multiple myeloma presenting as bilateral pleural effusion: a rare case presentation with review of literature
Multiple myeloma (MM) is a clonal proliferation of plasma cells that primarily affects the bone marrow and skeletal system. It mainly affects bone marrow although extramedullary tissues especially reticuloendothelial sys...
Comparative Immunohistochemical Analysis of Ki -67 in a Spectrum of Pediatric Solid Tumours
Introduction: The aim of this study is to analyse the clinicopathological profile of malignant pediatric solid tumours and also to evaluate the proliferative index using Ki-67 in a spectrum of these tumours. Materials an...
Fine needle aspiration cytology of thyroid lesions and classification as per the bethesda system
Aims The objective of this study was to analyse the thyroid cytology smears by The Bethesda System for Reporting Thyroid Cytopathology TBSRTC to determine the distribution of diagnostic categories and subcategories to an...