TEST OF ANTIOXIDANT ACTIVITY LEAVES OF SCAEVOLA TACCADA (GAERTN.) ROXB. USING DPPH (1, 1-DIPHENYL-2-PICRYLHYDRAZYL)
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2014, Vol 5, Issue 3
Abstract
Free radicals can caused damage cell and tissue which lead to various disease then needed antioxidant as radicals scavenging. The objective of this study was to determine antioxidants activity leaves of Scaevola taccada (Gaertn.) Roxb based to DPPH (1,1-diphenyl-2-picrilhydrazil) free radicals scavenging. The study was initiated with sample extraction by maceration used ethanol then partitioned with ethyl acetate. The obtained fraction tested to antioxidant activity DPPH radicals. Testing done in five series concentration is 50 ppm, 100 ppm, 200 ppm, 300 ppm and 400 ppm, which compared to antioxidant activity vitamin C and BHT (butylatedhydroxytoluene). Activity against free radicals is measured with at a spectrophotometer UV-VIS wavelength 517 nm and calculated value ES50. The result showed the value ES50 of a leaves ethyl acetate fraction of 1473 ppm while ES50 vitamin C and BHT of 9.054 ppm and 29.067 ppm, respectively. Therefore, obtained results showed that ethyl acetate fraction Scaevola taccada (Gaertn.) Roxb leaves has weak activity compared vitamin C and BHT as positive control with ES50 values respectively 1473.064 ppm, 9.054 ppm and 29.067 ppm.
Authors and Affiliations
Rahmawati , Safriani Rahman, Aulia Wati, Hendra Herman, Fauzan Arsyad
Keywords
Related Articles
- EP ID EP104794
- DOI 10.7897/2230-8407.050333
- Views 101
- Downloads 0
FORMULATION AND EVALUATION OF PRAVASTATIN SODIUM IMMEDIATE RELEASE TABLETS
The aim of present investigation is to develop immediate release formulation of Pravastatin sodium for oral drug delivery by using suitable concentration of superdisintegrants. Croscarmellose sodium (AC-DI-SOL), povidone...
POSSIBLE CARDIAC ADVERSE EFFECTS OF THERAPEUTIC DOSES OF MACROLIDE ANTIBIOTICS (AZITHROMYCIN AND CLARITHROMYCIN) IN HEALTHY JUVENILE RATS: BIOCHEMICAL ASSESSMENT
The drugs are associated with QT interval prolongation and cardiac dysrhythmias. This study was designed to determine whether or not a therapeutic oral dose of either azithromycin or clarithromycin administered for 5 or...
DEVELOPMENT AND VALIDATION OF A BIOANALYTICAL METHOD FOR DETERMINATION OF QUETIAPINE FROM HUMAN PLASMA
A simple high-performance liquid chromatographic (HPLC) method for the analysis of the antipsychotic drug quetiapine fumarate in human plasma has been developed. Zolpidem tartrate was employed as the internal standard (I...
DRUG NEOMYCIN RELEASE FROM CORDIA DICHOTOMA TRANSDERMAL FILM AND ANTIINFLAMMATORY ACTIVITY
Transdermal Films were prepared using 10 % w/v natural polymer (fruit gum) of Cordia dichotoma with different percentage of plasticizer (glycerin 0.10, 0.20 and 0.25 % w/v), same percentage of preservative (methyl para...
FORMULATION AND EVALUATION OF ORODISPERSIBLE TABLETS OF TRAMADOL HYDROCHLORIDE USING NOVEL CO-PROCESSED SUPERDISINTEGRANTS
In the present work, orodispersible tablets of Tramadol hydrochloride were prepared using novel co-processed superdisintegrants consisting of crospovidone and sodium starch glycolate in the different ratios (1:1, 1:2 &am...