THE ACTIVITY OF GLUTATHIONE SYNTHESIS AND CONJUGATION ENZYMES IN RAT HEPATOCYTES UNDER CONDITIONS OF LOW-PROTEIN DIET AND ACUTE LIVER INJURY
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2014, Vol 6, Issue 1
Abstract
The activity of enzymes of glutathione synthesis and conjugation were studied in rat hepatocytes under conditions of protein nutritional deprivation and acute acetaminophen -induced liver injury. Experimental animals were kept at a semisynthetic low-protein diet for 28 days to study the features of functioning of enzymes under conditions of protein nutritional deprivation. Acute toxic hepatitis modeled by acetaminophen administration per os at the rate of 1250 mg / kg (0,5 LD50) of the rats in the form of a suspension of 2% starch solution for 2 days. A significant decrease in to gluta-thione synthesis enzyme activity – γ-glutamyl cysteine synthase by 1.3 times compared to control group values was de-termined in hepatocytes under conditions of low-protein diet. A similar trend changes the dynamics of the studied enzymatic activity was observed in the rat group which modeled acute liver injury. However, the least values of the glu-tathione synthesis enzyme activity compared to control group values were fixed in rat hepatocytes hepatocytes under conditions of low-protein diet while and acetaminophen administration in toxic doses. We found decrease in to glutathione-S-transferase activity by 9 times compared to control group values in rat hepatocytes under conditions of protein deficiency. However, the acetaminophen administration in toxic doses is also accompanied by a significant decrease in the GST activity compared to control group values. As indicators glutathione-S- transferase activity was not significantly different in the rat group wich were acetaminophen administered on the background of protein defi-ciency and during the modeling of toxic hepatitis in conditions of nutrition, it should be noted that the acute liver cells injury is a determining factor in this case, regardless of the diet. A significant decrease in to level of reduced glutathione was determined in all experimental groups of rat hepatocytes takes place with a decrease in the glutathione-S-transferase activity. The research results indicate that level GSH was lower in 1.4 times than the control value under conditions of protein nutritional deprivation. At the same time the acetaminophen administration in toxic doses was associated with a decreased level of reduced glutathione in 1.6 times compared to control group values. However, synergistic effect of two negative factors - protein deficiency and drug - induced liver iniury were accompa-nied with a decrease in glutathione-S- transferase activity as well as with the decreased level of reduced glutathione in liver cells.
Authors and Affiliations
Г. КОПИЛЬЧУК, І. М. БУЧКОВСЬКА, Н. Л. БОРЩОВЕЦЬКА, Н. В. ЧОПИК
Keywords
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