THE ACTIVITY OF GLUTATHIONE SYNTHESIS AND CONJUGATION ENZYMES IN RAT HEPATOCYTES UNDER CONDITIONS OF LOW-PROTEIN DIET AND ACUTE LIVER INJURY
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2014, Vol 6, Issue 1
Abstract
The activity of enzymes of glutathione synthesis and conjugation were studied in rat hepatocytes under conditions of protein nutritional deprivation and acute acetaminophen -induced liver injury. Experimental animals were kept at a semisynthetic low-protein diet for 28 days to study the features of functioning of enzymes under conditions of protein nutritional deprivation. Acute toxic hepatitis modeled by acetaminophen administration per os at the rate of 1250 mg / kg (0,5 LD50) of the rats in the form of a suspension of 2% starch solution for 2 days. A significant decrease in to gluta-thione synthesis enzyme activity – γ-glutamyl cysteine synthase by 1.3 times compared to control group values was de-termined in hepatocytes under conditions of low-protein diet. A similar trend changes the dynamics of the studied enzymatic activity was observed in the rat group which modeled acute liver injury. However, the least values of the glu-tathione synthesis enzyme activity compared to control group values were fixed in rat hepatocytes hepatocytes under conditions of low-protein diet while and acetaminophen administration in toxic doses. We found decrease in to glutathione-S-transferase activity by 9 times compared to control group values in rat hepatocytes under conditions of protein deficiency. However, the acetaminophen administration in toxic doses is also accompanied by a significant decrease in the GST activity compared to control group values. As indicators glutathione-S- transferase activity was not significantly different in the rat group wich were acetaminophen administered on the background of protein defi-ciency and during the modeling of toxic hepatitis in conditions of nutrition, it should be noted that the acute liver cells injury is a determining factor in this case, regardless of the diet. A significant decrease in to level of reduced glutathione was determined in all experimental groups of rat hepatocytes takes place with a decrease in the glutathione-S-transferase activity. The research results indicate that level GSH was lower in 1.4 times than the control value under conditions of protein nutritional deprivation. At the same time the acetaminophen administration in toxic doses was associated with a decreased level of reduced glutathione in 1.6 times compared to control group values. However, synergistic effect of two negative factors - protein deficiency and drug - induced liver iniury were accompa-nied with a decrease in glutathione-S- transferase activity as well as with the decreased level of reduced glutathione in liver cells.
Authors and Affiliations
Г. КОПИЛЬЧУК, І. М. БУЧКОВСЬКА, Н. Л. БОРЩОВЕЦЬКА, Н. В. ЧОПИК
Keywords
Related Articles
- EP ID EP243653
- DOI -
- Views 103
- Downloads 0
Developmental traits in grassland and agricultural plants under the influence of ragweed
The problem of invasion and naturalization some of adventive species is very important in current moment. First of all, it concerns the species which influence to the human being. The Ambrosia artemisifolia L. is an one...
Biological educational museum сollection value of I. Horbachevsky Ternopil state medical university for learning about wildlife
The relationship between higher education and material collections of museums is deeply historic. Natural objects and their images are at the top of leading places among great variety of wildlife around us. That is the r...
Effects of imidazo[1,2-a]azepinie derivative ift_000281 on ATP-dependent potassium channels
In this work we have been experimentally tested the ability of imidazo[1,2-a]azepine derivative, compound under IFT_000281 code to activate the ATP-dependent potassium channels (KATP channels). To confirm or refute our h...
EXPRESSION OF PROTEIN PHOSPHATASE DUSP GENES IN SUBCUTANEOUS ADIPOSE TISSUE OF OBESE MEN WITH NORMAL AND IMPAIRED GLUCOSE TOLERANCE
We studied the expression of genes encodes the family of DUSP protein phosphatases in subcutaneous adipose tissue and isolated adipocytes of two groups of obese men: with normal and impaired glucose tolerance as compared...
BIOTECHNOLOGY FOR OBTAINING THE RECOMBINANT HEAT SHOCK PROTEIN (HSP-60) OF CHLAMYDIA TRACHOMATIS AND EVALUATION OF THE PERSPECTIVES OF ITS USE IN SEROLOGICAL DIAGNOSTICS
Identification of humoral immune responses to 60 kDa heat shock protein of Chlamydia trachomatis (HSP-60) is of great diagnostic value, as evidence of probable autoimmune process and risk of pathology of female reproduct...