The activity of N-acetyl-β-D-hexosaminidase A and B and β-glucuronidase in nasal polyps and hypertrophic nasal concha
Journal Title: Otolaryngologia Polska - Year 2014, Vol 68, Issue 1
Abstract
Nasal polyps and hypertrophic lower nasal conchae are common disorders of nasal cavity. The majority of etiopathogenetic theories indicate inflammatory background of polyps and hypertrophic concha. N-acetyl-b-D-hexosaminidase and b-glucuronidase are lysosomal exoglycosidases revealing accelerated activity in inflammatory processes. Aim: The aim of the study was to evaluate the catabolism of glycoconjugates in nasal polyps and hypertrophic nasal concha basing on the activity of N-acetyl-b-D-hexosaminidase (HEX) and b-glucuronidase (GLU). Material and methods: Material consisted of nasal polyps taken from 40 patients during polypectomy in patients with chronic rhinosinusitis with nasal polyps (CRSwNP) and hypertrophic lower nasal conchae taken from 20 patients during mucotomy. The activity of HEX, HEX A, HEX B and GLU in supernatant of homogenates of nasal polyps and hypertrophic lower nasal concha tissues has been estimated using colorimetric method. Results: Statistically significant decrease has been observed in concentration of the activity (per 1 mg of tissue) of HEX ( p < 0.05), HEX B ( p < 0.001) and specific activity (per 1 mg of protein) of HEX B ( p < 0.001) in nasal polyps tissue in comparison to hypertrophic lower nasal conchae tissue. Conclusions: Decrease in the activity and specific activity concentration of the majority of examined lysosomal exoglycosidases (increasing in inflammations) in comparison to hypertrophic lower nasal conchae suggests electrolytes disorders and questions the inflammatory background of nasal polyps.
Authors and Affiliations
Sylwia Chojnowska, Alina Minarowska, Napoleon Waszkiewicz, Beata Zalewska-Szajda, Elżbieta Gościk, Krzysztof Kowal, Ewa Olszewska, Emilia Konarzewska-Duchnowska, Łukasz Minarowski, Jerzy Ładny, Sławomir Szajda
Keywords
Related Articles
- EP ID EP52483
- DOI -
- Views 79
- Downloads 0
Spontaneous temporal bone meningo-encephalocele
Introduction. Spontaneous meningocele or meningoencephalocele of the temporal bone are very rare. Mostly they are result from otologic surgery or head trauma. Spontaneous meningoencephalocele may occur mostly in the epit...
Assessment of auditory processing in children with dyslalia
The objective of the work was to assess occurrence of central auditory processing disorders in children with dyslalia. Material and method: The material included 30 children at the age 7–9 years old being under long-term...
Ogólnopolski Program Profilaktyki i Wczesnego Wykrywania Nowotworów Głowy i Szyi
Każdego roku rozpoznaje się na świecie 550 tysięcy nowych zachorowań na nowotwory jamy ustnej i gardła oraz 160 tysięcy zachorowań na nowotwory krtani. Szacuje się, iż rocznie z powodu nowotworu głowy i szyi umiera 400 t...
Sekwencjonowanie genu GJB2 w populacji dzieci głuchych i z głębokim stopniem uszkodzenia odbiorczego słuchu
Wprowadzenie. Mutacje w genie GJB2 są najczęstszą przyczyną genetycznie uwarunkowanych niedosłuchów wrodzonych. Celem pracy była ocena częstości występowania mutacji w genie koneksyny 26 w grupie dzieci głuchych lub z gł...
Skala Warszawska A– E oceny endoskopowych zmian w krtani związanych z refluksem gardłowo-krtaniowym. Korelacja ze skalą patologii refluksowej (RFS)
Introduction: Changes associated with laryngopharyngeal reflux (LPR) are usually related to the posterior part of the larynx and called ‘‘posterior laryngitis’’. Pathologic reflux induced mucosal changes within the laryn...