The analysis of flavonoids in the flowering herbs of Carduus acanthoides L.
Journal Title: Current Issues in Pharmacy and Medical Sciences - Year 2013, Vol 26, Issue 1
Abstract
The present paper discusses the analysis of flavonoids in the methanol extract from the flowering herbs of Carduus acanthoides (L.), where flavonois were indentified by TLC and SPE HPLC. The quantitative analysis was performed using spectrophotometric Christ-Müller’s method. The samples containing flavonoids and those released after acid hydrolysis were investigated by 1D TLC on silica gel and polyamide. After purification by SPE, the samples were also analyzed by RP-HPLC. Apigenin-7-glucoside, luteolin-7-glucoside, kaempferol-3-rhamnoglucoside, kaempferol-3-glucoside, apigenin, luteolin and chlorogenic acid and p-coumaric acid were detected in the fractions of methanol extracts obtained from the plant. This method is very simple for the analysis and suitable for rapid screening of flavonoids in plants.
Authors and Affiliations
Małgorzata Kozyra, Kazimierz Głowniak
Keywords
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