The effects of COX2-inhibitors (etoricoxib and etodolac) on growth rate and mortality in Nile tilapia (Oreochromis niloticus)
Journal Title: Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society - Year 2011, Vol 4, Issue 5
Abstract
The non-steroidal anti inflammatory drugs represent one of the most commonly detected compounds in sewage treatment plant (STP) effluent and surface water with scarce information concerning possible ecotoxicological risks. As in mammals, COX has been shown to play a role in reproduction in fish. Since studies on human breast cancer cells showed that COXs-inhibitors decreased aromatase messenger ribonucleic acid (mRNA) expression at the transcriptional level we tested the effects of supplementation of COX2-inhibitors (etodolac and etoricoxib) in the diet of fry tilapia on growth rate and mortality during the crucial period of sexual differentiation. Highlight on etoricoxib pharmacokinetics was carried out by determination of etoricoxib in fish feces using reversed-phase High Performance Liquid Chromatography (RF-HPLC) with Evaporative Light Scattering and Photo Diode array detector (ELSD-PDA system). At an age of 8 days post-hatched, 30 genetically mixed population of Oreochromis niloticus larvae were stocked in duplicate, into 45 L aquariums in a closed system for six months. Treatments included 5 different experimental diets including, respectively, 0.5% etodolac, 1%etodolac, 2% etodolac, 0.5% etoricoxib, and 1% etoricoxib concentrations and one standard diet serving as control with two repeats for each group from 0.5% groups of diets. Fish were fed experimental diets for 8 weeks and were changed to control diet after. Another experiment was conducted using 10 adults mixed population stocked in triplicate in a closed system and treated as above. Growth rates (GR) were significantly affected with the highest growth rate obtained with the 0.5% etodolac. However, no increase or decrease in growth was observed in mixed adults population. GR increased with increasing concentrations with the highest GR in the aquarium treated with 2% etodolac, followed by 1% etodolac, but 1% etoricoxib showed a decreased GR compared to standard which could indicates a toxic potential toward fish at this concentration. No etoricoxib peak was detected on HPLC in feces samples which reflected, that, it was well absorbed by tilapias, extensively metabolized with no unchanged fraction excreted, or may undergo enter hepatic circulation, increasing further its toxic potential. No mortality was observed in adults mixed population.
Authors and Affiliations
Mutaz Al-Qutob, Iman Al-Hirsh, Tharwat Nashashibi
Keywords
Related Articles
- EP ID EP103157
- DOI -
- Views 114
- Downloads 0
Romanian legal instruments for implementing EU assistance in fishery.
In the present study the main components of the Fisheries Operational Programme (FOP) are presented. The implementation of these omponents aims to relaunch the Romanian fisheries sector on sustainable basis, given the f...
Mixed stock analysis (MSA) - a tool for proper management in fisheries
For natural environment, it is crucial to develop a fishery management plan, outlining conservation and restoration measures. From this point of view, if the fishery is managed as a single unit (stock), there is a huge p...
Freshwater snail Pomacea bridgesii (Gastropoda: Ampullariidae), life history traits and aquaculture potential
Investigations on the reproductive biology, life cycle and feeding habits of Pomacea bridgesii have been undertaken to assess its potential as a cultured species for the ornamental trade. The species is dioecious and, un...
Effect of EDTA on reducing tissue cadmium bioaccumulation and cadmium antagonism related to some mineral micro- and macronutrients in Prussian carp (Carassius gibelio).
EDTA is a chelating agent used deliberately in various fields (pulp and paper industry, detergents industry, food industry, medicine, biomedical labs) in order to sequester metal ions which have harmful effects in many p...
Development of primers for loop-mediated isothermal amplification of Philippine white spot syndrome virus isolates
Because there are currently no effective chemicals or drugs to completely treat bacterial and viral diseases, effective means of prevention such as early detection methods are continuously being explored and fully exhaus...