The electrophysiological characteristics of primary hamster cortical neurons with Mg2+-free external
Journal Title: Chinese Journal of Nervous and Mental Diseases - Year 2024, Vol 50, Issue 3
Abstract
[Objective] To investigate the electrophysiological characteristics of hamster primary cortical neurons by using the whole-cell patch-clamp recordings in a Mg2+-free cell model of epilepsy. [Methods] The cerebral cortex were isolated for primary neuron culture from neonatal Syrian hamsters 1-2 days after birth. After 12 days, primary neurons were exposed to normal external (Mg2+ group) or Mg2+-free external (Mg2+-free group) respectively for 3 hours, followed by a 24-hour incubation in normal culture medium. Excitatory postsynaptic currents (EPSC) and excitatory postsynaptic potentials (EPSP) were recorded in voltage clamp mode and current clamp mode using whole-cell patch-clamp recordings, respectively. [Results] Compared to Mg2+ group, the frequency of EPSC [(124.38±75.15) Hz vs. (33.93±22.32) Hz, P<0.001] (P<0.001) and EPSP [(37.05±38.37) Hz vs. (5.63±9.52) Hz, P<0.01] in primary cortical neurons from the Mg2+-free group increased significantly. Meanwhile, there were no statistically significant differences in the amplitude, area under the curve and half-width of EPSC and EPSP between the two groups (P>0.05). [Conclusion] The present study demonstrates an increase in excitability of primary hamsters cortical neurons after Mg2+ depletion, suggesting that these neurons can be utilized to use to create cell models of epilepsy.
Authors and Affiliations
Qiuyue SHEN, Nana LIU, Lili LIU, Yuwu JIANG, Xinlin. HOU
Keywords
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- EP ID EP750443
- DOI 10.3969/j.issn.1002-0152.2024.03.003
- Views 42
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