The Genetic Structure Features of the Pinus sylvestris L. Population in the Steppe Zone of European Russia
Journal Title: Lesnoy Zhurnal (Russian Forestry Journal) - Year 2024, Vol 2, Issue 2
Abstract
The article presents the results of studÑ of the genetic structure of Scots pine (Pinus sylvestris L.) growing in the ecologically favorable territory of the steppe zone of European Russia. 35âyear-old pine forest plantations (the Voronezh Region, Kantemirovskiy District, the 3rd bonitet class, forest site type â A1, a random sample, 60 trees) have been chosen as the object of the study. Genetic diversity has been examined on the basis of the microsatellite analysis of 18 SSR-loci (14 EST-SSR-loci and 4 nSSR-loci) and two isozymic loci (shikimate dehydrogenase and glutamate dehydrogenase â or Skdh and Gdh, respectively). It has been revealed that in the studied population, all the used microsatellite loci, with the exception of lw_isotig02842, are polymorphic. The average values of genetic variability calculated from the microsatellite loci have been obtained: the proportion of the polymorphic loci â 94.44 %, the average number of alleles per locus â 3.500, the average effective number of alleles â 2.466, the observed and expected heterozygosity â 0.209 and 0.493 respectively; the Wright fixation index â 0.577. In terms of the level of allelic diversity, the plantation is characterized by relatively lower values of genetic and statistical population parameters (the average observed and expected heterozygosity) compared to the natural forest-steppe populations of the Central Chernozem Region. A high positive Wright fixation index indicates the presence of an increased proportion of inbreeding in the steppe population trees. The reasons and possible nature of the revealed phenomenon are discussed. Based on the isoenzyme analysis, the genetic structure of the Skdh and Gdh loci is examined. The results of the analysis indicate a high frequency of the rapid allele Skdh-11 (82 %) and the embryonic semi-lethal allele Gdh-11 (22 %), which, apparently, can be considered as one of the mechanisms of the population adaptation to the conditions of a warmer and drier climate of the steppe region. A deficiency of glutamate dehydrogenase heterozygotes has been established. It has also been noted that the groups of EST-SSR-loci and nSSR-loci differ in the level of genetic variability and population structure. It has been shown that the combined use of different types of genetic markers makes it possible to obtain more complete and objective information on the distinctive features of the genetic structure of pine forests in the optimal and pessimal zones of the range. The studied 18 microsatellite loci can be used to assess the genetic diversity of Scots pine populations and trees in the steppe region of European Russia.
Authors and Affiliations
Irina I. KamalovaƗ, Мarta Yu. Peturenko, Аlina P. Degtyareva, Nina F. Kuznetsova, Natal’ya I. Vnukova
Keywords
Related Articles
- EP ID EP734366
- DOI 10.37482/0536-1036-2024-2-49-64
- Views 62
- Downloads 0
Climate Change and Its Impact on Adaptation and Intraspecific Variability of Conifer Species of the European North of Russia
Current climate change is affecting forests and requires a specific forest management strategy. The review aims to analyze the impact of observed and projected climate change on the tree species adaptation response with...
The Potential Impact of Climate Change on the Distribution of Norway Spruce (Picea abies Karst.) in Bosnia and Herzegovina
As forests in Bosnia and Herzegovina cover 2,904,600 ha or 56.7 % of its total area, and since the meteorological data analysis for the period 1961–2014 shows that the mean annual temperature maintains a continuous rise,...
Ash Content of Containerboard. Problems and Solutions
This article covers the study of the nature of ash content of containerboard from waste paper, as well as fractional composition of ash. With increasing use of waste paper with white top layer and deterioration of condit...
Dynamic Behavior of Liquid Flow Rate from Nozzles in Jet Scrubbers of Pulp Production
It is shown that the modern development of pulp production technology is associated with the development of gas-liquid systems equipment. Such equipment provides the main technological processes of pulp cooking and regen...
Zonality of Forest Fuels and Their Pyrogenic Transformation in Pine Forests of Central Siberia
Fires in the forests of Siberia occur annually and cause enormous damage to natural resources. Therefore, studies of the natural forest fire hazard, one of the components of which are forest fuels, are relevant. The rese...