The impact of addition of reduced glutathione and bovine serum albumin to the extender on the qualitative indexes of the frozen-thawed ram sperm
Journal Title: Acta Scientiarum Polonorum Zootechnica - Year 2014, Vol 13, Issue 3
Abstract
The study revealed the optimal concentration of reduced glutathione and bovine serum albumin in the lactose-yolk-tris-citrate-glycerine diluent in ram–sires sperm cryopreservation. It was shown that the addition of 5 μM of reduced glutathione and 15 mg · ml-1 of bovine serum albumin into the lactose-yolk-tris-citrate-glycerine diluent contributes to the increase in frozen–thawed sperm activity by 11.0%, its resistance to cryopreservation by 6.0%, intact acrosome by 9.5%, sperm surviving rate by 23.9%, absolute survival indicator by 21.8%, sperm kinetic indicators: VCL – by 14.3%, VAP – by 12.5%, VSL – by 15.4%, LIN – by 0.4%, STR – by 2.2%, as well as fertilization rate by 12.0%. These results prove the positive effect of the addition of bioactive substances into the diluent on the qualitative features of frozen–thawed sperm.
Authors and Affiliations
Chrystyna Grymak, Stakh Vovk
Keywords
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