The impact of culture media on the endothelial viability of corneas
Journal Title: Moldovan Medical Journal - Year 2018, Vol 61, Issue 4
Abstract
Background: The complexity of cryopreservation and its potential to damage the endothelium make it so that it is rarely used in routine eye banking, except for occasional, clinically urgent transplants, where the main objective is to save the eye. The culture medium was the method of choice in the Eye Bank. Material and methods: The study group was divided into 3 subgroups. For penetrating keratoplasty and anterior lamellar keratoplasty, corneas taken up to 24 hours, with a number greater than 2000 endothelial cells per mm2 , are preferably used. Results: In the first group we found: weakly edematous epithelium; thin, transparent stroma; thin Descemet’s membrane; transparent endothelial layer, endothelial cell density greater than 2800 cells/mm2. The corneas of the second group presented: edematous, but with uncompromised integrity epithelium; slightly edematous, transparent stroma; slightly folded Descemet’s membrane; intact endothelial layer and an average of 2600 cells/mm2. Corneas of the third group: evidently edematous epithelium, with exfoliations in some areas and Bowman’s membrane detachment; considerable edema of stroma in all layers; pronounced folds of Descemet’s membrane; interrupted endothelial layer along the outline of the folds. Conclusions: The age of the donor and the preservation time are important factors that influence corneas in culture media and determine the state of endothelial cells. Although the number of endothelial cells usually decreases with age, there are still many corneas from donors over 80 years of age who meet the minimum criteria for transplantation.
Authors and Affiliations
Adrian Cociug, Olga Macagonova, Valeriu Cusnir Jr, Vitalie Procopciuc, Viorel Nacu
Keywords
Related Articles
- EP ID EP478857
- DOI 10.5281/zenodo.2222291
- Views 369
- Downloads 1
Interests in knowledge and assistance of epilepsy
Background: Many problems with differential treatment of epilepsy require further clarification. As far as we are concerned, we have developed therapeutic recommendations which, in our opinion, demonstrated to be effecti...
Modern approach to pediatric asthma
Background: The article reviews aims to promote the communication between scientists engaged in basic research and clinicians working with children, in order to better understand the mechanism and work together towards a...
Prevalence of primary headaches in adolescents
Background: The aim of this study was to estimate overall prevalence of primary headaches and prevalence of migraine (MG) and tension-type headache (TTH) among adolescents in the Republic of Moldova. Metetial and methods...
Selection of antihypertensive drugs from the perspective of clinical pharmacology
Background: The rational use of medicines remains one of the most important directions of public health, especially in socio-medical diseases, including arterial hypertension. Material and methods: The study involved 28...
Correlation between spinal nerves, anterolateral abdominal wall muscle tone and inguinal hernia
Background: The complete diagnosis of pathomorphological disorders in case of diseases of the spine determines the choice of effective treatment, pathogenetically argued in various diseases which, according to modern cla...