The protective effects of Crocin on testopathy in fat-fed and streptozotocin-treated diabetic rats: An experimental study
Journal Title: International Journal of Reproductive BioMedicine - Year 2019, Vol 17, Issue 2
Abstract
Background: Male hypogonadism is associated with type II diabetes mellitus due to testicular dysfunction. Medicinal plants have received considerable attention for the management of diabetes and its complications. Objective: The aim of present study was to evaluate the anti-diabetic and protective influence of Crocin on testopathy in diabetic rats. Materials and Methods: In this experimental study, type II of diabetes mellitus was induced by high-fat diet and low dose of streptozotocin. Male Wistar rats (8 weeks, 150–200 gr, 18 rats; n= 6 per group) were divided into a control group (standard diet), diabetic group (streptozotocin+high-fat diet), and treatment group (High-fat diet+streptozotocin+Crocin at 20 mg/kg/day, i.p. for 60 days). After 60 days, animals were euthanized, testis and epididymis were dissected, and weights of testes and sperm count were analyzed. Hematoxylin-eosin-stained was done for histopathological examination. Blood samples were collected for the assessment of serum glucose and cholesterol. Results: High-fat diet and streptozotocin significantly increased the serum glucose and cholesterol levels as compared to the control group (p≤ 0. 001). Moreover, there was a significant decrease in the weight of right (p= 0.008) and left testes (p≤ 0. 001) and also the total sperm count (p= 0.023) in the diabetic group compared with the control group. Current results also identified that type II diabetes mellitus induced degeneration in the morphology of seminiferous tubules. Application of Crocin could significantly decrease serum glucose and cholesterol levels (p= 0.003). Furthermore, Crocin treatment significantly increased the weight of the right (p= 0.026) and left (p= 0.014) testes and total sperm count (p= 0.000). Also, Crocin could attenuate the pathological changes of the testes in the treatment group. Conclusion: Present findings concluded that Crocin treatment improved diabetic testopathy and impairment of seminiferous tubules induced by high-fat diet and streptozotocin.
Authors and Affiliations
Mohammad Saeed Mirzaee, Mohammad Ehsan Bayatpoor, Shima Shahyad, Mohammad Ali Mohammadi, Zahra Bahari
Keywords
Related Articles
- EP ID EP642582
- DOI -
- Views 92
- Downloads 0
Maternal serum uric acid level and maternal and neonatal complications in preeclamptic women: A cross-sectional study
Background: Preeclampsia is associated with maternal and neonatal complications. It has been indicated that increased uric acid might have a predictive role on preeclampsia. Objective: We aimed to investigate the relatio...
The effect of endometrial injury on first cycle IVF/ICSI outcome: A randomized controlled trial
Background: Implantation remains a limiting step in IVF/ICSI. Endometrial injury isa promising procedure aiming at improving the implantation and pregnancy rates after IVF/ICSI. Objective: The aim of this study was to e...
Bibliometric mapping and clustering analysis of Iranian papers on reproductive medicine in Scopus database (2010-2014)
Background: To meet the future challenges in the field of reproductive medicine in Iran, better understanding of published studies is needed. Bibliometric methods and social network analysis have been used to measure the...
Protective effect of hydroalcoholic extract of Teucrium polium on diabetes-induced testicular damage and serum testosterone concentration
Background: Diabetes has an adverse effect on spermatogenesis by rising oxidative stress. Objective: The aim of this study was to investigate the effect of Teucrium Polium extract administration on spermatogenesis and te...
Association study of Glutathione S-Transferase polymorphisms and risk of endometriosis in an Iranian population
Background: Endometriosis influenced by both genetic and environmental factors. Associations of glutathione S-transferases (GSTs) genes polymorphisms in endometriosis have been investigated by various researchers; howeve...