Time Effect Storage Artifacts of Anticoagulant EDTA on Peripheral Blood Cells
Journal Title: Journal of Medical Science And clinical Research - Year 2015, Vol 3, Issue 5
Abstract
Aims & Objectives: Exclude the EDTA induced storage artifacts .Identify and exclude the misinterpretation of peripheral blood smears examination .Identify the EDTA induced RBC & WBC morphological storage artifacts. Identify the EDTA induced platelets related artifacts. Material & Methods: Blood was collected in a sterile EDTA containing tube and processed following our established laboratory protocol .A complete blood counting including HB%,PCV, Red cell indices ,platelet count and total white cell count and differential was done by Automated blood cell counter and peripheral blood smear examination then a sterile EDTA containing blood sample tube stored at room temperature. The all cell count indices including RBC,WBC count with differential along with morphological storage artifacts and platelet count with storages artifacts, was further confirmed by manual oil immersion smear study method. Peripheral smears study was done with field A and B stain and leishman stain. Conclusion: EDTA cause the various storage artefacts encountered on peripheral blood smear examination when smear prepared from prolong stored sterile EDTA containing blood sample tube at room temperature. EDTA cause RBC, WBC morphological artifacts and platelets related artifacts .These artifacts lead to various misinterpretation of peripheral blood smear examination so exclude them.
Authors and Affiliations
Dr. Ramu Thakur
Keywords
Related Articles
- EP ID EP211599
- DOI -
- Views 102
- Downloads 0
Comparison of the haemodynamic responses to laryngoscopy and intubation with MacIntosh and McCoy laryngoscopy
Background: Stress response to laryngoscopy and tracheal intubation have a profound influence on the circulatory parameters and the intracranial pressure. The mean increase in arterial pressure of the order of 20-25 mmHg...
Mystery of Auto-Haemotherapy
Auto-haemotherapy is the mystery that medicine had missed in the past. It is the power that can stimulate the immune system of the body in order to re-adjust all the body’s functions including the endocrinologial system,...
Clinical, Bacteriological Profile and Management Practices in Culture Positive Urinary Tract Infection in Children
Introduction: Urinary tract infection (UTI) is seen in approximately 3-5% of girls and 1 % of boys. UTI can be the first presentation in 30% of children with urinary tract anomalies. Failure to identify these patients ca...
Dengue Fever Patients May Develop Proteinuria and Hematuria – A Report of 18 Patients
Present study relates to the results of urine analysis of 18 patients with Dengue fever. Dengue antigen (Ag) detection was done in sera of patients with febrile illness by ELISAusing monoclonal murine anti-NS1 antibody....
A Study of Serum Cystatin C in Chronic Kidney Disease Patients Undergoing Hemodialysis
Introduction: CKD has become a major public health problem (1). According to Dash and Agarwal (2006) (2) the prevalence of CKD in India accounts to around 0.8% of the total population which would be around 7.85 million o...